Abstract
Mouse monoclonal antibodies to CD19 detect an antigenic determinant expressed exclusively on the surface of B lymphocytes, and have previously been shown to be potentially useful therapeutic reagents for human B cell lymphoma. We report the production and characterization of a mouse/human chimeric antibody, cCD19, with potent in vivo antitumour activity. The genes encoding the variable domains for heavy (VH) and light (VL) chains were subcloned into eukaryotic expression vectors containing human constant region genes (IgG1 and kappa), and co-transfected into non-secreting Sp2/0 mouse myeloma cells. Intraperitoneal administration of cCD19 produced inhibition of growth of subcutaneous CD19+ Sultan human B lymphoma tumours in scid/scid mice. When the antibody was administered 18 and 20 days after subcutaneous tumour inoculation, an approximately 30% reduction in tumour size was noted by day 29. cCD19 faithfully mimicked the in vitro binding characteristics of mCD19 as (a) the chimeric antibody was shown by flow cytometry to bind exclusively to cell lines that expressed CD19, (b) cCD19 was able to inhibit the binding of mCD19 on CD19+ cells completely and (c) the affinity of binding of the two antibodies was not significantly diffe...Continue Reading
References
Jul 1, 1978·Immunochemistry·P L EyC R Jenkin
May 16, 1992·Lancet·B FaliniG Pizzolo
Apr 22, 1992·International Journal of Cancer. Journal International Du Cancer·P RivaG Franceschi
Jan 1, 1992·Cancer Immunology, Immunotherapy : CII·R HandgretingerD Niethammer
Jan 1, 1991·Cancer Immunology, Immunotherapy : CII·A HekmanC J Melief
Dec 1, 1991·Immunology and Cell Biology·H ZolaY Kurosawa
Jun 1, 1990·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·G E GoodmanK E Hellström
Aug 1, 1989·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·O W PressK A Krohn
May 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·R OrlandiG Winter
Jun 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·A F LoBuglioM B Khazaeli
Dec 17, 1988·Lancet·G HaleH Waldmann
Mar 24, 1988·Nature·L RiechmannG Winter
Oct 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·Y Haruta, B K Seon
Aug 9, 1974·Nature·N S Harris
Aug 12, 1993·The New England Journal of Medicine·M S KaminskiG M Butchko
Aug 1, 1993·Cancer Immunology, Immunotherapy : CII·A J RowlandI F McKenzie
Jun 26, 1993·Lancet·C M LockwoodH Waldmann
Feb 1, 1994·Cancer·S F VervoordeldonkI C Slaper-Cortenbach
Feb 1, 1993·Journal of Immunotherapy with Emphasis on Tumor Immunology : Official Journal of the Society for Biological Therapy·L M WeinerD G Haller
Jul 9, 1993·Science·P A TrailK E Hellström
Aug 1, 1993·British Journal of Cancer·V HirdA A Epenetos
Jan 1, 1993·American Journal of Surgery·B M PetersenJ W Harmon
May 5, 1962·Nature·W M HUNTER, F C GREENWOOD
Citations
Nov 9, 2006·Springer Seminars in Immunopathology·Thomas F TedderYan Xiu
May 5, 1998·Molecular Immunology·I C NicholsonA W Hohmann
Jan 5, 2002·Veterinary Immunology and Immunopathology·Sylvain De GuiseDavid A Ferrick
Oct 12, 2005·Proceedings of the National Academy of Sciences of the United States of America·Norihito YazawaThomas F Tedder
Aug 1, 2007·Cancer Immunology, Immunotherapy : CII·Julia StieglmaierWijnand Helfrich
Jul 21, 2005·British Journal of Haematology·Joerg BruenkeGeorg H Fey
Jul 5, 2005·Expert Opinion on Investigational Drugs·S MukerjeeM C Glassy
Feb 23, 2011·Cancer Letters·Christian KellnerGeorg H Fey
Mar 10, 2016·British Poultry Science·C Sánchez-GarcíaV R Gaudioso
Jun 29, 2001·Transplant Infectious Disease : an Official Journal of the Transplantation Society·T J Walsh, A H Groll
Mar 15, 2006·Journal of Immunotherapy·Karin BarbinGeorg H Fey
May 15, 2007·Leukemia·M SchwemmleinG H Fey
Dec 26, 2008·Blood·Jonathan ZalevskyChris E Lawrence
Aug 1, 1996·Leukemia & Lymphoma·L M PilarskiA R Belch
Feb 4, 2009·Protein Engineering, Design & Selection : PEDS·Markus KüglerGeorg H Fey
Jun 22, 2005·International Immunology·Jun YanMark J Mamula
Apr 6, 2006·Cancer Research·Maty TzukermanKarl Skorecki
May 27, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Camelia I SpiridonEllen S Vitetta
Jan 12, 2005·Protein Expression and Purification·Dipankar DasMavanur R Suresh