In vitro and in vivo assessment of oral autologous artificial connective tissue characteristics that influence its performance as a graft

Tissue Engineering. Part a
Marta R Fontanilla, Lady Espinosa

Abstract

Several studies have evaluated proteins secreted by fibroblasts comprising skin substitutes, finding that they are secreted in combinations and concentrations that promote wound healing. However, assessment of proteins secreted by oral fibroblasts forming a part of oral substitutes is scarce. In our previous work, collagen type-I scaffolds (CSs) and autologous artificial connective tissue (AACT) were produced and implanted in rabbit oral lesions, evidencing that AACT outperforms CS. The present work determined the secreted factor profile of AACT in the time of grafting as well as that of the AACT embedded in the clot. It also evaluated the proliferation and viability of AACT fibroblasts to establish the dwell time of these cells in the grafted area. Finally, it assessed whether CS, AACT, and clot-embedded AACT increase fibroblast recruitment induced by a fibrin clot, because the cell migratory response has been associated with the wound-healing outcome. We found that some of the factors secreted by AACT fibroblasts are significantly different from those secreted by clot-embedded AACT fibroblasts. Also, that the profile of proteins secreted by AACT fibroblasts and clot-embedded AACT fibroblasts is different from already reported...Continue Reading

References

Jun 1, 1986·The Journal of Cell Biology·P KnoxC S Rimmer
Jul 1, 1997·The Cleft Palate-craniofacial Journal : Official Publication of the American Cleft Palate-Craniofacial Association·M Fujioka, T Fujii
Sep 2, 1999·The New England Journal of Medicine·A J Singer, R A Clark
Aug 17, 2000·Springer Seminars in Immunopathology·A JelaskaJ H Korn
Feb 28, 2001·Diabetes, Obesity & Metabolism·J N MansbridgeG K Naughton
Mar 30, 2001·The Journal of Biological Chemistry·N AshidaT Kita
Nov 8, 2001·Plastic and Reconstructive Surgery·V K Rumalla, G L Borah
Dec 24, 2003·Journal of Artificial Organs : the Official Journal of the Japanese Society for Artificial Organs·Kentaro Kubo, Yoshimitsu Kuroyanagi
Feb 18, 2004·Biomaterials·Abdoelwaheb El-GhalbzouriMaria Ponec
Apr 29, 2004·Plastic and Reconstructive Surgery·Richard D PriceHarshad A Navsaria
Sep 15, 2004·Tissue Engineering·M GriffithsH Navsaria
Apr 22, 2005·Journal of Oral Rehabilitation·J D Raguse, H J Gath
Sep 28, 2005·Artificial Organs·Kentaro Kubo, Yoshimitsu Kuroyanagi
Nov 10, 2006·Clinical Oral Implants Research·Sebastian SauerbierRainer Schmelzeisen
Feb 15, 2007·The Journal of Investigative Dermatology·Sabine A EmingJeffrey M Davidson
Apr 28, 2007·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·C LuitaudM Rouabhia
May 31, 2007·The British Journal of Dermatology·T WongH Navsaria
Oct 20, 2007·Nature Protocols·Jan W Slot, Hans J Geuze
Nov 1, 2007·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Sander W SpiekstraSusan Gibbs
Dec 20, 2007·Oral and Maxillofacial Surgery Clinics of North America·Kenji Izumi, Stephen E Feinberg
May 16, 2008·Nature·Geoffrey C GurtnerMichael T Longaker
Feb 3, 2009·Journal of Artificial Organs : the Official Journal of the Japanese Society for Artificial Organs·Naoto YamadaYoshimitsu Kuroyanagi
May 19, 2009·Journal of Oral and Maxillofacial Surgery : Official Journal of the American Association of Oral and Maxillofacial Surgeons·Qin XuStephen E Feinberg
Jul 7, 2009·Annual Review of Cell and Developmental Biology·Frederick Grinnell, W Matthew Petroll
Sep 23, 2009·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Joyce W WongLari Häkkinen
Jan 19, 2010·Orthodontics & Craniofacial Research·J LiuJ W Von den Hoff
Sep 25, 2010·Nature Reviews. Immunology·Lydia Sorokin

❮ Previous
Next ❯

Citations

Nov 6, 2018·Drug Delivery and Translational Research·Liliana Gil-CifuentesMarta R Fontanilla

❮ Previous
Next ❯

Methods Mentioned

BETA
protein-array
antibody array
light microscopy
enzyme-linked immunosorbent assay

Software Mentioned

Gene Tools File

Related Concepts

Related Feeds

Blood Clotting Disorders

Thrombophilia includes conditions with increased tendency for excessive blood clotting. Blood clotting occurs when the body has insufficient amounts of specialized proteins that make blood clot and stop bleeding. Here is the latest research on blood clotting disorders.

Allogenic & Autologous Therapies

Allogenic therapies are generated in large batches from unrelated donor tissues such as bone marrow. In contrast, autologous therapies are manufactures as a single lot from the patient being treated. Here is the latest research on allogenic and autologous therapies.

© 2021 Meta ULC. All rights reserved