In vitro and in vivo characteristics of stem cells derived from the periodontal ligament of human deciduous and permanent teeth

Tissue Engineering. Part a
Je Seon SongJae-Ho Lee

Abstract

In many studies, adult stem cells have been found in human periodontal ligament (PDL), but in most cases they were found in the permanent teeth. The aim of the present study was to characterize stem cells from the PDL of deciduous teeth (dPDLSCs) and compare them with those from the PDL of permanent teeth (pPDLSCs). Stem cell markers were examined by a flow cytometric analysis. The results of in vitro differentiation into adipogenic and osteogenic lineages were analyzed by histochemical staining and quantitative reverse transcription-polymerase chain reaction (RT-PCR). The results of in vivo transplantation were analyzed by histological staining, immunohistochemical staining, and quantitative RT-PCR. There were no significant differences in the proliferation rate, cell cycle distribution, expressions of stem cell markers such as Stro-1 and CD146, or in vitro differentiation. The pPDLSC transplants made more typical cementum/PDL-like tissues and expressed more cementum/PDL-related genes (CP23 and collagen XII) than did the dPDLSC transplants. Together, these results suggest that pPDLSCs are better candidates for use in reconstructing periodontium.

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Citations

Nov 26, 2013·Cellular and Molecular Life Sciences : CMLS·Wing-Fu LaiHan-Sung Jung
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Jul 13, 2021·World Journal of Stem Cells·Aline QueirozMarinella Holzhausen

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Methods Mentioned

BETA
flow cytometry
PCR
PCRs
dissection

Software Mentioned

FCSExpress
SPSS

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