In vitro and in vivo characterization of silk fibroin/gelatin composite scaffolds for liver tissue engineering

Journal of Digestive Diseases
Zhao YangXin Min Zhou

Abstract

To investigate the cytotoxicity of silk fibroin/gelatin (SF/G) composite scaffolds in vitro as well as their biocompatibility and degradation in vivo. The proliferation and relative growth rate of human hepatic QZG cells grown on different blends of two-dimensional (2-D) SF/G scaffolds were assessed using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Flow cytometry was used to evaluate apoptotic rate of QZG cells on different blends of 2-D SF/G scaffolds. The effect of silk protein materials on cell growth was observed by scanning electron microscopy. Three-dimensional (3-D) SF/G scaffolds of three different ratios (diameter 10 mm, thickness 1 mm) were implanted into subcutaneous pockets on male Sprague-Dawley (SD) rats. On the 7th, 14th and 30th day post-implantation, the rats were sacrificed. The scaffold area including the surrounding tissues was retrieved. Hematoxylin and eosin staining was performed for observation under a light microscope. Significant cell attachment and proliferation on the SF/G scaffolds were observed. As the increased gelatin concentration, SF/G scaffolds became more amenable to cell adhesion. After the subcutaneous implantation of the SF/G scaffolds in SD rats, immunolo...Continue Reading

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Citations

Jul 6, 2014·Journal of Controlled Release : Official Journal of the Controlled Release Society·Kati-Sisko VellonenArto Urtti
Apr 1, 2015·Proceedings of the National Academy of Sciences of the United States of America·Alexandra BerdichevskiDror Seliktar
Jan 25, 2020·Advanced Healthcare Materials·Alain da Silva MoraisRui L Reis
Mar 19, 2015·Biomedical Materials·Luyuan YangJian R Lu
Aug 21, 2013·Macromolecular Bioscience·Paola TaddeiGianluca Ciardelli
Jun 28, 2014·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Nuno H C S SilvaArmando J D Silvestre
Dec 5, 2016·Biochemistry. Biokhimii︠a︡·A Y ArkhipovaM M Moisenovich
Jul 10, 2019·Bioengineering·Shicheng YeKerstin Schneeberger
May 31, 2018·Technology and Health Care : Official Journal of the European Society for Engineering and Medicine·Artūras KilikevičiusRimantas Kačianauskas
Nov 10, 2020·International Journal of Biological Macromolecules·Resmi RajalekshmiAnugya Bhatt

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