In vitro and in vivo characterization of glycoprotein C-deleted infectious laryngotracheitis virus

The Journal of General Virology
Sophia PavlovaWalter Fuchs

Abstract

Infectious laryngotracheitis is an important respiratory disease of chickens that is caused by an alphaherpesvirus [infectious laryngotracheitis virus (ILTV); Gallid herpesvirus 1]. As herpesvirus envelope glycoproteins are main targets of the humoral host immune response, they are of particular interest for development of vaccines, as well as of diagnostic tools. The conserved, N-glycosylated envelope protein gC has been identified as a major surface antigen of ILTV. To study the function of gC, we now isolated a gC-deleted ILTV recombinant as well as a gC rescuant after co-transfection of permissive chicken cells with virion DNA and transfer plasmids containing engineered subgenomic fragments. Like other alphaherpesvirus homologues, ILTV gC proved to be non-essential for replication. ILTV-DeltagC exhibited delayed penetration kinetics and slightly reduced plaque sizes in cultured chicken cells, whereas virus titres were not reduced significantly compared with wild-type or gC-rescued virus. In vivo studies revealed that ILTV-DeltagC is attenuated in chickens. However, infection with high doses of ILTV-DeltagC was still fatal for approximately 20 % of the animals, whereas wild-type or gC-rescued ILTV led to 50 % mortality. Inte...Continue Reading

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Citations

Apr 25, 2013·Avian Pathology : Journal of the W.V.P.A·Alireza MahmoudianGlenn F Browning
May 31, 2013·Avian Pathology : Journal of the W.V.P.A·Mauricio J C CoppoJoanne M Devlin
Apr 20, 2011·BMC Genomics·Sang-Won LeeJoanne M Devlin
Apr 10, 2013·Developmental and Comparative Immunology·Mauricio J C CoppoJoanne M Devlin
Jun 11, 2016·The Journal of General Virology·Maricarmen GarciaG H Schneiders
Nov 1, 2013·World Journal of Virology·Shan-Chia Ou, Joseph J Giambrone

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