In vitro and in vivo (cyto)toxicity assays using PVC and LDPE as model materials

Journal of Biomedical Materials Research. Part a
E A E Van TienhovenW H De Jong

Abstract

The choice for a biomaterial is partly based on the outcome of (cyto)toxicity assays. The rationales behind the selection of certain parameters, such as cell lines, controls, and animals, were evaluated using a positive and a negative control, and one experimental sample designed to induce intermediate toxicity. Extraction and direct contact assays were performed using human epidermal keratinocytes and mouse fibroblasts and mouse epithelial cells. Cell survival was measured with the tetrazolium salt (MTT) reduction assay. In addition, local implantation studies were performed in mice and rats. The positive control induced a high degree of toxicity in all in vitro tests performed, indicating that the toxicity observed in the direct contact assay was due to in situ extraction of toxic components. In the direct contact assay the negative control tested on the mouse fibroblasts resulted in a significant reduction of cell survival. No decrease in cell survival was found using the experimental sample. Subcutaneous implantation studies in mice showed that the positive control material induced a severe degeneration in mice. However, in rats just minimal alterations were noted. The experimental material induced moderate responses only i...Continue Reading

References

Mar 1, 1988·The Journal of Cell Biology·P BoukampN E Fusenig
Apr 1, 1984·Journal of Biomedical Materials Research·T N Salthouse
Apr 1, 1993·Biomaterials·G CiapettiA Pizzoferrato
May 1, 1996·Dental Materials : Official Publication of the Academy of Dental Materials·C T HanksZ Sun
Jun 5, 1998·Proceedings of the Institution of Mechanical Engineers. Part H, Journal of Engineering in Medicine·C J KirkpatrickM Otto
Dec 9, 1993·Clinical Materials·A PizzoferratoL Savarino
Mar 29, 2000·Journal of Biomedical Materials Research·J A CadéeW E Hennink
Sep 28, 2000·Journal of Biomedical Materials Research·I M KhouwM J van Luyn
Jan 5, 2002·Journal of Biomedical Materials Research·Kameha R KiddStuart K Williams
Aug 31, 2002·Biomolecular Engineering·C J KirkpatrickK Peters
Feb 13, 2003·Journal of Biomedical Materials Research. Part a·Harmke BonnemaMarja J A van Luyn
May 20, 2003·Artificial Organs·Sizue O RogeroAurea S Cruz
May 30, 2003·Australian Endodontic Journal : the Journal of the Australian Society of Endodontology Inc·G GambariniM Castagnola
Sep 25, 2003·Journal of Endodontics·Jean Camps, Imad About
Nov 19, 2003·Journal of Biomedical Materials Research. Part a·Bai-Shuan LiuShan-Hui Hsu
Feb 6, 2004·Journal of Biomedical Materials Research. Part a·Xioadong WangGang Chen
Apr 2, 2004·Journal of Biomedical Materials Research. Part a·LeeAnn O BaileyFrancis W Wang
Jun 29, 2004·Applied Microbiology and Biotechnology·E Lavik, R Langer
Jul 17, 2004·Annual Review of Biomedical Engineering·Buddy D Ratner, Stephanie J Bryant
Sep 7, 2004·Journal of Materials Science. Materials in Medicine·T G Van KootenR Eloy
Jan 26, 2005·Journal of Biomedical Materials Research. Part a·Sandy JusteJean-Pierre Hallé
Mar 15, 2005·Biomaterials·Yanchun LiuGlenn D Prestwich

❮ Previous
Next ❯

Citations

Jan 23, 2010·Applied Microbiology and Biotechnology·Regine EiblDieter Eibl
Mar 26, 2013·International Journal of Pharmaceutics·U Gianfranco SpizzirriFrancesca Iemma
Mar 4, 2014·Materials Science & Engineering. C, Materials for Biological Applications·Maryam NabiyouniSarit B Bhaduri
May 23, 2014·Journal of Materials Science. Materials in Medicine·Finosh Gnanaprakasam Thankam, Jayabalan Muthu
Sep 3, 2013·Advanced Healthcare Materials·Gayoung ParkKyung-Mi Lee
Jun 13, 2017·Sensors·Cindy SchaudeGerhard J Mohr
Jul 12, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Milena PopovaIoanna Chinou
Mar 6, 2007·Journal of Biomedical Materials Research. Part a·Menno L W KnetschLeo H Koole
May 29, 2007·Journal of Biomedical Materials Research. Part a·Wim H De JongWim E Hennink
Mar 16, 2019·Materials·José Henrique de Lima CavalcantiSergio Alexandre Gehrke
May 23, 2018·Nanomaterials·Riccardo GuazzoBarbara Zavan
May 4, 2013·Journal of Biomaterials Applications·Martin Sebastian WalterHåvard Jostein Haugen
Jan 31, 2012·ASAIO Journal : a Peer-reviewed Journal of the American Society for Artificial Internal Organs·Hua KunYang Xiubin
Dec 15, 2020·The Science of the Total Environment·Arifur RahmanJaroslav Slobodnik

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.