In vitro and in vivo effects of Ginkgo biloba extract EGb 761 on seeded Schwann cells within poly(DL-lactic acid-co-glycolic acid) conduits for peripheral nerve regeneration

Journal of Biomaterials Applications
Shan-Hui HsuFang-Tsun Lin

Abstract

This study investigated the effects of Ginkgo biloba (EGb 761) extract on seeded Schwann cells within poly(DL-lactic acid-co-glycolic acid) (PLGA) conduits by in vitro and in vivo trials for peripheral nerve regeneration. The seeding efficiency of Schwann cells in serum-deprived culture medium, which simulated the environment of mechanical trauma on an injured nerve site, was improved by adding different dosages of EGb 761 (0, 1, 10, 20, 50, 100, 200 microg/mL). The analytical results showed enhanced cell attachment and survival, reduced LDH release and increased MTT values, particularly in the range 10-100 microg/mL. The PLGA nerve conduits seeded with Schwann cells (6 x 10(3) cells) and filled with gelatin containing EGb 761 (0, 10, 50, 100 microg/mL) were implanted to 10-mm right sciatic nerve defects in rats. Autograft was performed as another control. Electromyography was assessed based on the motor unit action potential (MUAP) and fibrillation potential (Fib) at 2, 4, and 6 weeks during all periods. The specimens of the experimental and control groups were harvested for histological analysis at 6 weeks after surgery. The Fib was found to gradually decay, and the MUAP was found not to be present until 4 weeks after surgery...Continue Reading

References

Mar 1, 1990·Plastic and Reconstructive Surgery·S E Mackinnon, A L Dellon
Jan 1, 1990·Annual Review of Neuroscience·J W Fawcett, R J Keynes
Nov 1, 1990·World Journal of Surgery·H Millesi
May 26, 1995·Biochemical Pharmacology·I MaitraL Packer
Sep 1, 1994·Experimental Neurology·T J MahalikG P Owens
Aug 1, 1993·Planta medica·C BrunoE Bombardelli
May 1, 1996·Biomaterials·N Nicoli AldiniR Giardino
Feb 1, 1996·Biomaterials·S WoerlyA R Harvey
Apr 1, 1997·Journal of Reconstructive Microsurgery·J K TerzisP K Thanos
Oct 1, 1993·Journal of Applied Biomaterials : an Official Journal of the Society for Biomaterials·C P TountasH V Mendenhall
Jan 3, 2001·Muscle & Nerve. Supplement·D W Zochodne
Feb 22, 2001·Free Radical Biology & Medicine·K IshigeY Sagara
Mar 12, 2002·The European Journal of Neuroscience·Eric G FinePatrick Aebischer
Sep 6, 2002·Brain Research·Satoru YoshiiMasao Akagi
Oct 21, 2003·Journal of Biomedical Materials Research. Part a·Satoru YoshiiMasao Akagi
Oct 31, 2003·Biomaterials·Yueh-Sheng ChenChun-Hsu Yao

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Citations

Oct 2, 2012·International Journal of Pediatric Otorhinolaryngology·Chul Ho JangCheol Hee Choi
Jul 19, 2011·Journal of Controlled Release : Official Journal of the Controlled Release Society·Amit PabariAlexander M Seifalian
Jun 15, 2007·Journal of the Peripheral Nervous System : JPNS·Lukas A PfisterBruno Gander
Mar 26, 2010·Journal of Biomedical Materials Research. Part a·Bing-Cang LiJian-Mei Chen
Sep 23, 2009·Experimental Neurology·Xu JiangSing Yian Chew
Aug 18, 2009·International Review of Neurobiology·Hede YanWilliam C Lineaweaver
Sep 12, 2017·Journal of Biomedical Materials Research. Part a·Travis A PrestJonathan Cheetham
Sep 26, 2006·Annals of Plastic Surgery·Michael B ChenWilliam C Lineaweaver
Aug 19, 2007·Journal of Materials Science. Materials in Medicine·Bin LiuXiao-Kun Li
Nov 2, 2005·Biotechnology and Bioengineering·Shan-hui HsuChun-Jung Chen
Apr 4, 2007·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Chen-Jung ChangShih-Kuang Hsu

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