In vitro and in vivo effects of three different Mitragyna speciosa korth leaf extracts on phase II drug metabolizing enzymes--glutathione transferases (GSTs).

Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry
Juzaili AziziSharif Mahsufi Mansor

Abstract

In the present study, we investigate the effects of three different Mitragyna speciosa extracts, namely methanolic, aqueous and total alkaloid extracts, on glutathione transferase-specific activity in male Sprague Dawley rat liver cytosol in vitro and in vivo. In the in vitro study, the effect of Mitragyna speciosa extracts (0.01 to 750 microg/mL) against the specific activity of glutathione transferases was examined in rat liver cytosolic fraction from untreated rats. Our data show concentration dependent inhibition of cytosolic GSTs when Mitragyna speciosa extract was added into the reaction mixture. At the highest concentration used, the methanolic extract showed the highest GSTs specific activity inhibition (61%), followed by aqueous (50%) and total alkaloid extract (43%), respectively. In in vivo study, three different dosages; 50, 100 and 200 mg/kg for methanolic and aqueous extracts and 5, 10 and 20 mg/kg for total alkaloid extract were given orally for 14 days. An increase in GST specific activity was generally observed. However, only Mitragyna speciosa aqueous extract with a dosage of 100 mg/kg showed significant results: 129% compared to control.

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Citations

Apr 30, 2011·Journal of Medical Toxicology : Official Journal of the American College of Medical Toxicology·Friedrich G KappMaren Hermanns-Clausen
Jul 6, 2014·BMC Complementary and Alternative Medicine·Rejoice MoyoStanley Mukanganyama
Nov 26, 2015·Journal of Psychoactive Drugs·Marc T SwoggerZach Walsh
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Jan 1, 2013·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Ee Lin LimMei Lan Tan
Apr 20, 2016·International Journal of Molecular Sciences·Flaminia PantanoEnrico Marinelli
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Jan 11, 2020·Drugs·Jonathan Schimmel, Richard C Dart
Jan 30, 2020·Pain and Therapy·Steven C EastlackAlan D Kaye
May 10, 2017·Frontiers in Psychiatry·Dimy Fluyau, Neelambika Revadigar
Oct 28, 2019·Preventive Medicine·Jack E HenningfieldEdward J Cone
Jul 25, 2018·Journal of Natural Products·Saroopa P SamaradivakaraL M Viranga Tillekeratne

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