In vitro and in vivo effects of unsaturated fatty acids on Schistosoma mansoni and S. haematobium lung-stage larvae

The Journal of Parasitology
Hatem TallimaRashika El-Ridi

Abstract

Incubation of Schistosoma mansoni lung-stage larvae in 90% corn oil for 6 hr was shown to elicit exposure of their, otherwise masked, apical membrane antigens to binding of anti-schistosome antibodies in the indirect membrane immunofluorescence test (IF). The possibility that unsaturated fatty acids (FA) are responsible for this effect was herein supported by IF data on ex vivo lung-stage larvae of S. mansoni and S. haematobium incubated for 1/2-2 hr with 80% corn oil, 50% olive oil, or 10-20 microM arachidonic acid. Treatment with unsaturated FA followed by filipin staining for cholesterol visualization indicated that unsaturated FA do not induce exposure of schistosomular surface membrane antigens via extraction of surface membrane cholesterol. Evidence using inhibitors and stimulators of neutral sphingomyelinase suggested that unsaturated FA perhaps activate worm tegument-bound neutral sphingomyelinase, leading to sphingomyelin hydrolysis and changes in surface membrane fluidity. Larval apical membrane antigens are, thus, allowed to diffuse freely in the plane of the membrane and bind specific antibodies in IE Excessive sphingomyelin hydrolysis might explain why high FA concentrations or long incubation periods eventually le...Continue Reading

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Citations

May 19, 2010·Antimicrobial Agents and Chemotherapy·Rashika El RidiOmar M Fahmy
Apr 29, 2011·Molecular and Biochemical Parasitology·Hatem TallimaRashika El Ridi
Mar 28, 2006·Cytokine·Rashika El RidiHatem Tallima
May 23, 2006·International Immunopharmacology·Rashika El RidiHatem Tallima
Apr 12, 2016·Biochimica Et Biophysica Acta. General Subjects·Rashika El RidiFederica Migliardo
Sep 1, 2013·Journal of Advanced Research·Rashika El Ridi, Hatem Tallima
Mar 5, 2014·Biochemical and Biophysical Research Communications·Federica MigliardoRashika El Ridi
Feb 20, 2007·Memórias do Instituto Oswaldo Cruz·Maira G R PittaMônica C P A Albuquerque
Jan 28, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Bruno J NevesPedro V L Cravo
Dec 14, 2017·Future Medicinal Chemistry·Eloi M LagoJosué de Moraes

Methods Mentioned

BETA
light microscopy
antisense oligonucleotides

Related Concepts

Antigens, Helminth
Surface Antigens
Epicholesterol
Dietary Oils
Enzyme Activation
Fatty Acids, Unsaturated
Filipin IV
Cricetus
Tadpole
Lung

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