PMID: 8611625Apr 10, 1996Paper

In vitro and in vivo gene transfer to pulmonary cells mediated by cationic liposomes

Biochimica Et Biophysica Acta
E FortunatiM Scarpa

Abstract

Cationic liposomes have been proposed as alternative to adenovirus in the treatment of cystic fibrosis lung disease. Therefore, we have investigated the efficiency of two lipid mixtures in mediating gene transfer in in vitro and in vivo models. The cationic lipid DOTMA (N-(1-(2,3(dioleyloxy)propyl)-n,n,n-trimethylammoniumchloride++ +) and DOGS (dioctadecylamidoglycylspermine) were used in combination with the neutral lipid DOPE (dioleoylphosphatidylethanolamine). The relative transfection efficiencies of the two cationic liposomes were tested using the bacterial beta-galactosidase (lacZ) and the firefly luciferase genes. Gene expression was detected in both cell limes and primary culture of rhesus monkey airway epithelium after transfection with plasmid DNA complexed with DOGS/DOPE or DOTMA/DOPE. Transfection efficiency of both types of lipids was higher in the mouse fibroblast 3T3 cell line as compared to human carcinoma A549 cells and primary epithelial cultures. Administration of DNA-liposome complexes via intratracheal instillation resulted in expression of the lacZ and luciferase marker gene in the mouse airways. In vivo transfection mediated by both types of liposomes were proven to be far less efficient than adenovirus t...Continue Reading

References

Jan 26, 1989·Nature·P L Felgner, G M Ringold
Mar 25, 1989·Nucleic Acids Research·G R MacGregor, C T Caskey
Sep 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·J P BehrJ Perez-Mutul
Mar 1, 1993·Experimental Lung Research·A BoutB J Scholte
Jan 1, 1994·Human Gene Therapy·M VitadelloS Schiaffino
May 10, 1994·Proceedings of the National Academy of Sciences of the United States of America·Y YangJ M Wilson
Jul 1, 1993·DNA and Cell Biology·F BarthelJ P Behr
Apr 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·J Herz, R D Gerard

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Citations

Dec 10, 1999·The Annals of Thoracic Surgery·M YanoG A Patterson
Jul 7, 1997·Advanced Drug Delivery Reviews·R NivenJ Smith
Jun 6, 2000·Advanced Drug Delivery Reviews·R K Scheule, S H Cheng
Mar 4, 1997·Proceedings of the National Academy of Sciences of the United States of America·N OudrhiriP Lehn
Dec 28, 1999·Human Gene Therapy·K SchughartH V Kolbe
May 13, 2003·Tissue Engineering·Stephen M WarrenMichael T Longaker
Jul 8, 2005·Human Gene Therapy·Léa DesigauxBruno Pitard
Jun 22, 2005·Journal of Aerosol Medicine : the Official Journal of the International Society for Aerosols in Medicine·A SteimerC-M Lehr
Jul 21, 2004·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Colman K ByrnesJohn W Harmon
Oct 27, 2011·Journal of Drug Delivery·Abdelbary M A ElhissiAntony D'Emanuele
Jul 1, 2010·Therapeutic Delivery·Corinna PfeiferCarsten Rudolph
Apr 5, 2002·Genome Research·Jae-Hong KoTomomasa Watanabe
Apr 23, 2014·The Journal of Gene Medicine·Chia-Ling ChanCyrus R Safinya
Nov 17, 2004·Journal of Controlled Release : Official Journal of the Controlled Release Society·Shubiao ZhangZongshi Li
Aug 14, 2012·Journal of Invertebrate Pathology·Rahul Hepat, Yonggyun Kim
May 26, 2005·Journal of Cystic Fibrosis : Official Journal of the European Cystic Fibrosis Society·Uta GriesenbachUNKNOWN UK Cystic Fibrosis Gene Therapy Consortium
Feb 29, 2000·Biophysical Journal·M T KennedyR C MacDonald
May 26, 2004·Biochimica Et Biophysica Acta·Ye Zhang, Thomas J Anchordoquy
Jul 21, 2001·Molecular Membrane Biology·S Audouy, D Hoekstra
Aug 7, 2007·Cytogenetic and Genome Research·T Watanabe
Dec 19, 2002·The Journal of Experimental Zoology·Mario ValenzuelaPedro Esponda
Mar 1, 2000·Gene Therapy·R G JenkinsS L Hart
Oct 8, 2011·Genome Biology and Evolution·Eviatar Nevo
Aug 20, 2019·Journal of Liposome Research·Pirthi Pal SinghRavinder Kodipyaka
Dec 10, 2002·American Journal of Physiology. Lung Cellular and Molecular Physiology·Meera Nanjundan, Fred Possmayer
Jul 15, 2000·The Journal of Biological Chemistry·V PectorJ M Ruysschaert
Sep 23, 2000·American Journal of Physiology. Lung Cellular and Molecular Physiology·H SaitoH Tomoike
Mar 4, 2009·Journal of Liposome Research·Sarantis GiatrellisGeorge Nounesis

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