In Vitro and In Vivo Immunomodulatory Activity of Okra (Abelmoschus esculentus L.) Polysaccharides

Journal of Medicinal Food
Huricha ChenFengyang Wang

Abstract

Crude okra (Abelmoschus esculentus L.) polysaccharide (RPS) was obtained by water extraction and alcohol precipitation. Three purified fractions of RPS, designated RPS-1, RPS-2, and RPS-3, were fractioned by diethylaminoethyl (DEAE)-cellulose chromatography. Their molecular weights, monosaccharide compositions, infrared (Fourier transform infrared [FT-IR]) spectra, and nuclear magnetic resonance (NMR) spectra were analyzed. Their immunomodulatory activity was evaluated with an in vitro cell model (RAW264.7 cells). In vivo immunomodulatory activity of RPS-2 was evaluated in normal and cyclophosphamide-induced immunosuppressed mice. The results showed that the molecular weights of RPS-1, RPS-2, and RPS-3 were 600, 990, and 1300 kDa, respectively. RPS-1 and RPS-2 were mainly composed of galactose, rhamnose, galacturonic acid, and glucuronic acid, while RPS-3 was mainly composed of galactose, rhamnose, galacturonic acid, glucuronic acid, and glucose. FT-IR and NMR spectrum data indicated a rhamnogalacturonan I characteristic of polysaccharide. Both RPS and its purified fractions RPS-1, RPS-2, and RPS-3 significantly increased RAW264.7 cell proliferation, nitric oxide (NO) production, inducible nitric oxide synthase (iNOS) expressio...Continue Reading

References

Aug 15, 1991·Analytical Biochemistry·T M Filisetti-Cozzi, N C Carpita
Jan 20, 1998·Trends in Biochemical Sciences·B Mayer, B Hemmens
Jun 30, 1998·Biulleten' eksperimental'noĭ biologii i meditsiny·N I ShereneshevaT I Klochkova
Apr 4, 2001·Der Hautarzt; Zeitschrift für Dermatologie, Venerologie, und verwandte Gebiete·R W BirkS Goerdt
Nov 5, 2002·Critical Reviews in Oncology/hematology·A H KlimpT Daemen
Nov 12, 2002·Journal of Ethnopharmacology·Ilhan GürbüzNalan Akyürek
Mar 20, 2004·Journal of Agricultural and Food Chemistry·Christian LengsfeldAndreas Hensel
May 26, 2005·International Immunopharmacology·Kun Yeong Lee, Young Jin Jeon
Nov 9, 2005·International Immunopharmacology·Igor A SchepetkinMark T Quinn
Jan 24, 2006·Journal of Ethnopharmacology·Ava RhuleDavid M Shepherd
Jan 24, 2006·International Immunopharmacology·Igor A Schepetkin, Mark T Quinn
May 9, 2009·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·O E AdelakunM Van de Venter
Dec 17, 2009·Phytotherapy Research : PTR·Muriel VayssadeMarie-Danielle Nagel
Jul 26, 2011·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Jianming GuoYi You
Sep 23, 2011·Cellular Immunology·Lu-Hang ZhaoDeng-Po Weng
Apr 1, 2012·Saudi Pharmaceutical Journal : SPJ : the Official Publication of the Saudi Pharmaceutical Society·S I Alqasoumi

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Citations

Oct 15, 2019·Critical Reviews in Food Science and Nutrition·Dongmei WuShiguo Chen
Mar 30, 2020·Phytotherapy Research : PTR·Danial EsmaeilzadehHossein Hosseinzadeh
Nov 8, 2018·Advances in Pharmacological Sciences·Sri Puji Astuti WahyuningsihWin Darmanto
Jan 13, 2021·Journal of the Science of Food and Agriculture·Baoyi XiongZhaojun Xian
Feb 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Abd Elmoneim O ElkhalifaSyed Amir Ashraf
Feb 26, 2021·International Journal of Biological Macromolecules·Selina Mawunyo Ayivi-TosuhGuiyun Wang

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Methods Mentioned

BETA
Gel Imaging
enzyme-linked immunoabsorbent assay
ion-exchange chromatography
nuclear magnetic resonance
ELISA

Software Mentioned

Bandscan
SAS

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