In vitro and in vivo inhibition of beta-carotene dioxygenase activity by canthaxanthin in rat intestine

Archives of Biochemistry and Biophysics
Pascal GrolierV Azais-Braesco

Abstract

beta-Carotene dioxygenase catalyzes the conversion of provitamin A carotenoids to vitamin A in mammalian tissues. Whether the enzyme can also cleave non-provitamin A carotenoids to retinoid analogs with biological activities is still unclear. We investigated (i) substrate specificities of beta-carotene dioxygenase toward provitamin A and non-provitamin A carotenoids and (ii) potential antagonistic effects of non-provitamin A carotenoids on beta-carotene conversion to vitamin A. Provitamin A substrates were 8 to 23% as active as beta-carotene. No polar metabolites were detected with canthaxanthin or zeaxanthin as substrates; these compounds efficiently inhibited the beta-carotene cleavage reaction by 71 and 40%, respectively. Kinetic studies indicated mixed inhibition for canthaxanthin (Ki = 1.6 microM) and non-competitive for zeaxanthin (Ki = 7.8 microM), suggesting that both compounds do not interact significantly with the active site of the enzyme. In vivo, dietary combinations of canthaxanthin and beta-carotene resulted in lower liver levels of both carotenoids and vitamin A and in a higher beta-carotene/vitamin A ratio as compared to groups supplemented with the compounds separately. This supports the view that canthaxanthi...Continue Reading

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Citations

Mar 20, 2010·The Journal of Nutrition·Janet A NovotnyAnne C Kurilich
Jul 6, 2010·Archives of Biochemistry and Biophysics·Georg LietzGerald Rimbach
Sep 25, 2007·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Noriaki YamaguchiSachiko Takase
Mar 12, 2017·Nutrients·Patrick Borel, Charles Desmarchelier
Nov 5, 2013·The Journal of Biological Chemistry·Carlo dela SeñaEarl H Harrison
Feb 13, 2019·The Proceedings of the Nutrition Society·Torsten BohnPatrick Borel
Aug 19, 2010·Applied Microbiology and Biotechnology·Yeong-Su Kim, Deok-Kun Oh
Feb 27, 1999·Nutrition Reviews·H van den Berg
Mar 30, 2005·The Journal of Nutrition·Emmanuelle ReboulMarie-Josèphe Amiot
May 5, 2016·The Journal of Biological Chemistry·Carlo Dela SeñaEarl H Harrison
Apr 5, 2000·Nutrition Reviews·W A PryorC L Rock
Oct 1, 2011·Molecular Nutrition & Food Research·Patrick Borel

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