In vitro and in vivo interaction between cisplatin and topotecan in ovarian carcinoma systems

Cancer Chemotherapy and Pharmacology
S RomanelliF Zunino

Abstract

Topotecan, a camptothecin analogue, is a specific inhibitor of topoisomerase I approved for use in the treatment of patients with refractory ovarian carcinoma. The drug's mechanism of action suggests a potential efficacy of drug combinations incorporating DNA-damaging agents. In an attempt better to define a rational basis for drug combination we examined the effect of topotecan on the cytotoxicity and antitumor activity of cisplatin in an ovarian carcinoma system growing in vitro and in vivo as a tumor xenograft. The in vitro cell system included a cisplatin-sensitive cell line, IGROV-1, and a cisplatin-resistant subline, IGROV-1/Pt0.5, which is characterized by p53 mutation and loss of normal function of the wild-type gene of the parental cell line. This cell system was chosen since the cell sensitivity to DNA-damaging agents appears to be dependent on p53 gene status. Cytotoxicity was assessed by the growth inhibition assay using different schedules: (a) a 1-h period of cisplatin exposure followed by a 24-h topotecan treatment and (b) a 1-h period of simultaneous exposure to cisplatin and topotecan. In the case of the sequential schedule, an additive interaction was observed in IGROV-1 and IGROV-1/Pt0.5 cells. When the simul...Continue Reading

Citations

Nov 16, 2004·Cancer Chemotherapy and Pharmacology·Camilla PalumboAndrea Modesti
Dec 10, 2002·Cancer Treatment Reviews·M CrulJ H M Schellens
Feb 13, 2001·Critical Reviews in Oncology/hematology·W ZoliD Amadori
Jan 30, 2010·Molecular Pharmaceutics·Vincent J Venditto, Eric E Simanek
Jun 9, 2005·Natural Product Research·Dharmarajan SriramTanushree Ratan Bal
Jan 25, 2013·International Journal of Breast Cancer·Denzel R WoodeRobert Clarke
Feb 24, 2012·International Journal of Nanomedicine·Puiyan LeeKenneth K Y Wong
Aug 2, 2008·Journal of Thoracic Oncology : Official Publication of the International Association for the Study of Lung Cancer·Morten SorensenSeppo Wang Langer
Nov 16, 2012·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·F ZanaboniF Raspagliesi
Sep 22, 2009·Critical Reviews in Oncology/hematology·Domenica LorussoGiovanni Scambia
Mar 5, 2014·Journal of Cellular Physiology·Chiara ArientiAnna Tesei
May 18, 2005·Cancer Letters·Kenneth K W ToSteve C F Au-Yeung
Oct 29, 2005·Gynecologic Oncology·Howard S HochsterUNKNOWN New York Gynecologic Oncology Group (and phase II consortium) and the Eastern Cooperative Oncology
Jul 23, 2008·Gynecologic Oncology·Christos A PapadimitriouUNKNOWN Hellenic Co-operative Oncology Group
Jan 30, 2007·Expert Opinion on Pharmacotherapy·Leslie M Randall-Whitis, Bradley J Monk
May 25, 2005·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Harry J LongUNKNOWN Gynecologic Oncology Group Study
Sep 1, 2006·Cancer Research and Treatment : Official Journal of Korean Cancer Association·Joo Young JungNoe Kyoung Kim
Mar 7, 2006·International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society·L FusoI Vergote
Dec 13, 2017·Oncotarget·Ayesha N Shajahan-HaqRobert Clarke
Feb 16, 2010·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Ruchi NehraRobert Clarke
Oct 12, 2000·Medical and Pediatric Oncology·C Rodriguez-GalindoP J Houghton

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.