In vitro and in vivo investigation of thermosensitive chitosan hydrogels containing silica nanoparticles for vaccine delivery

European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences
Sarah GordonSarah Hook

Abstract

In this work silica nanoparticles (SNP) containing the model antigen ovalbumin (OVA) were incorporated into a thermosensitive chitosan hydrogel, and the resulting formulation investigated for its potential to act as a particulate sustained release vaccine delivery system. OVA-loaded SNP and chitosan hydrogels containing OVA-loaded SNP were prepared and characterised in vitro, and examined for their ability to elicit OVA-specific immune responses in vivo. Optimised SNP were found to be approximately 300nm in size with a moderate level of heterogeneity, a highly negative zeta potential, and an entrapment efficiency of approximately 7%. A porous particulate structure was indicated both by electron microscopy and a rapid release of fluorescently-labelled OVA (FITC-OVA) from SNP. Following successful incorporation of SNP into chitosan hydrogels, the release of both soluble and SNP-associated antigen from gel systems was quantified. Approximately 16% of total protein was released in a particulate form over a 14-day period, while approximately 35% was released as soluble antigen. Gel-based systems containing SNP-associated or soluble antigen in the presence or absence of the adjuvant Quil A (QA) demonstrated an ability to stimulate bo...Continue Reading

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Citations

Sep 23, 2014·Progress in Polymer Science·Minh Khanh Nguyen, Eben Alsberg
Apr 2, 2014·Immunology and Cell Biology·Silke NeumannSarah Hook
May 14, 2011·Expert Opinion on Drug Delivery·Zhenqing Li, Jianjun Guan
Dec 9, 2014·Acta Biomaterialia·Justin R AdamsSurya K Mallapragada
Sep 14, 2013·Nanomedicine : Nanotechnology, Biology, and Medicine·Xu WuJulia Xiaojun Zhao
Feb 25, 2015·Immunology and Cell Biology·Andrew J HightonRoslyn A Kemp
Jul 6, 2016·Pharmaceutical Research·Sharan Bobbala, Sarah Hook
Nov 24, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Pariksha J KondiahViness Pillay
Jan 15, 2019·Artificial Cells, Nanomedicine, and Biotechnology·Abbas HajizadeAyyoob Arpanaei
Sep 29, 2018·Food Science and Biotechnology·Jeong Bin MinHyeon Gyu Lee
Nov 25, 2020·Emerging Topics in Life Sciences·Sharan Bobbala, Sarah Hook
Mar 5, 2019·Current Medicinal Chemistry·Shuang YuKai Zhao

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