In vitro and in vivo metabolism of ochratoxin A: a comparative study using ultra-performance liquid chromatography-quadrupole/time-of-flight hybrid mass spectrometry

Analytical and Bioanalytical Chemistry
Shupeng YangZhanhui Wang

Abstract

Ochratoxin A (OTA) is a mycotoxin that frequently contaminates a wide variety of food and feedstuffs. The metabolism of OTA greatly affects fate and toxicity in humans and animals, because of its possible carcinogenic character (International Agency for Research on Cancer (IARC), group 2B). To completely characterize the metabolites of OTA, the metabolism of OTA in liver microsomes of rats, chickens, swine, goats, cows, and humans was investigated using ultra-performance liquid chromatography-quadrupole/time-of-flight hybrid mass spectrometry (UPLC-Q/TOF-MS). In addition, an in vivo comparative metabolism study of OTA was performed among rats and chickens after oral administration of OTA. As a result, a clear metabolic profile of OTA in different species was proposed, and a total of eight metabolites were identified, of which three hydroxylated metabolites at the phenylalanine moiety were discovered for the first time (preliminarily identified as 9'-OH-OTA, 7'-OH-OTA, and 5'-OH-OTA). Considerable amounts of 7'-OH-OTA were detected in different species' liver microsomes, especially in chickens and humans. Moreover, the metabolism of OTA in chickens was elucidated for the first time in the present study. The 7'-OH-OTA proved to b...Continue Reading

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Citations

Oct 28, 2015·Toxins·Alexandra H Heussner, Lewis E H Bingle
Jul 8, 2016·Toxins·Frantisek MalirJakub Toman
Oct 24, 2020·Toxins·Zhi Hung LohMary T Fletcher
Dec 3, 2020·Toxins·Beatriz Arce-LópezElena González-Peñas
Sep 1, 2018·Comprehensive Reviews in Food Science and Food Safety·Arnau VidalMarthe De Boevre
Nov 17, 2021·Journal of Agricultural and Food Chemistry·Melvin S SamuelEthiraj Selvarajan

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