In vitro and in vivo ocular biocompatibility of electrospun poly(ɛ-caprolactone) nanofibers

European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences
Gisele Rodrigues Da SilvaFrancine Behar-Cohen

Abstract

Biocompatibility is a requirement for the development of nanofibers for ophthalmic applications. In this study, nanofibers were elaborated using poly(ε-caprolactone) via electrospinning. The ocular biocompatibility of this material was investigated. MIO-M1 and ARPE-19 cell cultures were incubated with nanofibers and cellular responses were monitored by viability and morphology. The in vitro biocompatibility revealed that the nanofibers were not cytotoxic to the ocular cells. These cells exposed to the nanofibers proliferated and formed an organized monolayer. ARPE-19 and MIO-M1 cells were capable of expressing GFAP, respectively, demonstrating their functionality. Nanofibers were inserted into the vitreous cavity of the rat's eye for 10days and the in vivo biocompatibility was investigated using Optical Coherence Tomography (OCT), histology and measuring the expression of pro-inflammatory genes (IL-1β, TNF-α, VEGF and iNOS) (real-time PCR). The OCT and the histological analyzes exhibited the preserved architecture of the tissues of the eye. The biomaterial did not elicit an inflammatory reaction and pro-inflammatory cytokines were not expressed by the retinal cells, and the other posterior tissues of the eye. Results from the b...Continue Reading

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Citations

Jan 9, 2017·Colloids and Surfaces. B, Biointerfaces·Abbas AkhgariAli Nokhodchi
Apr 26, 2016·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·Nathan A HotalingKapil Bharti
Aug 23, 2019·Polymers·Courtney LynchViness Pillay
Feb 23, 2017·Pharmaceutical Research·Gabriella M Fernandes-CunhaFrancine Behar-Cohen
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Nov 12, 2020·Biomaterials Science·Zoe HancoxFarshid Sefat
Apr 4, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Asmaa M Abd El-AzizSherif H Kandil
May 28, 2019·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Gisele Rodrigues Da SilvaFrancine Behar-Cohen
Jun 12, 2017·ACS Biomaterials Science & Engineering·Yangzi TianYubing Xie

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