PMID: 8590897Jan 1, 1995Paper

In vitro and in vivo production of interleukin-6 induced by muramyl peptides and lipopolysaccharide in normal dogs

Cancer Biotherapy
F ShiE G MacEwen

Abstract

Interleukin-6 (IL-6) is a multifactorial cytokine produced by many cells including monocytes and macrophages in the immune-stimulated host. We measured IL-6 activity induced by muramyl dipeptide (MDP) and lipopolysaccharide (LPS) in vitro and by liposome-encapsulated muramyl tripeptide-phosphatidylethanolamine (L-MTP-PE) in vivo in normal dogs. Adherent mononuclear cells were cultured with MDP, LPS, or MDP plus LPS for various time periods. After incubation, culture supernatants were collected and assayed for IL-6 activity. Sera from dogs following L-MTP-PE administration were also evaluated for IL-6 activity. IL-6 activity both in supernatants and sera was measured using a 7TD1 bioassay. Significantly elevated IL-6 activity could be measured as early as 2 hours after mononuclear cells were exposed to MDP, LPS, or MDP plus LPS. IL-6 activity induced by LPS was greater than that induced by MDP, and the combination of MDP and LPS induced the greatest increase in IL-6 activity. Serum IL-6 activity was elevated within 3 to 4 hours post L-MTP-PE administration and subsequently declined to pretreatment level at 24 hours post injection. Neutralization of supernatant and serum IL-6 activity was not achieved with goat or rabbit anti-rec...Continue Reading

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Citations

Dec 7, 2000·Cancer Investigation·D M Vail, E G MacEwen
Aug 5, 2015·Cell and Tissue Research·Grace AmpemTamás Röszer
Jan 1, 1996·The Veterinary Clinics of North America. Small Animal Practice·E G MacEwen, I D Kurzman
Apr 29, 1998·The Veterinary Clinics of North America. Small Animal Practice·S A Kruth

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