In vitro and in vivo testing of glucose-responsive insulin-delivery microdevices in diabetic rats

Lab on a Chip
Michael ChuYu Sun

Abstract

We have developed glucose-responsive implantable microdevices for closed-loop delivery of insulin and conducted in vivo testing of these devices in diabetic rats. The microdevices consist of an albumin-based bioinorganic membrane that utilizes glucose oxidase (GOx), catalase (CAT) and manganese dioxide (MnO(2)) nanoparticles to convert a change in the environmental glucose level to a pH stimulus, which regulates the volume of pH-sensitive hydrogel nanoparticles and thereby the permeability of the membrane. The membrane is integrated with microfabricated PDMS (polydimethylsiloxane) structures to form compact, stand-alone microdevices, which do not require tethering wires or tubes. During in vitro testing, the microdevices showed glucose-responsive insulin release over multiple cycles at clinically relevant glucose concentrations. In vivo, the microdevices were able to counter hyperglycemia in diabetic rats over a one-week period. The in vitro and in vivo testing results demonstrated the efficacy of closed-loop biosensing and rapid response of the 'smart' insulin delivery devices.

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Citations

Jan 19, 2016·Chemical Society Reviews·Mahdi KarimiMichael R Hamblin
Aug 29, 2013·Macromolecular Rapid Communications·Natascha HotzWilfried Weber
Apr 5, 2015·Biosensors & Bioelectronics·Stefan MrossMichael Kraft
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Apr 30, 2013·Polymers·Seila SelimovićAli Khademhosseini
Aug 20, 2019·Advanced Materials·Jinqiang WangZhen Gu
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Sep 25, 2020·Pharmaceutical Research·Stephanie FuchsMinglin Ma
Feb 26, 2021·International Journal of Nanomedicine·Chen LiKeke Wang
Dec 29, 2020·Chemical Reviews·Stephanie FuchsMinglin Ma

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