In vitro and intracellular activities of frog skin temporins against Legionella pneumophila and its eukaryotic hosts.

Scientific Reports
Alexandre CrépinJulien Verdon

Abstract

Temporin-SHa (SHa) is a small cationic host defence peptide (HDP) produced in skin secretions of the Sahara frog Pelophylax saharicus. This peptide has a broad-spectrum activity, efficiently targeting bacteria, parasites and viruses. Noticeably, SHa has demonstrated an ability to kill Leishmania infantum parasites (amastigotes) within macrophages. Recently, an analog of SHa with an increased net positive charge, named [K3]SHa, has been designed to improve those activities. SHa and [K3]SHa were both shown to exhibit leishmanicidal activity mainly by permeabilization of cell membranes but could also induce apoptotis-like death. Temporins are usually poorly active against Gram-negative bacteria whereas many of these species are of public health interest. Among them, Legionella pneumophila, the etiological agent of Legionnaire's disease, is of major concern. Indeed, this bacterium adopts an intracellular lifestyle and replicate inside alveolar macrophages likewise inside its numerous protozoan hosts. Despite several authors have studied the antimicrobial activity of many compounds on L. pneumophila released from host cells, nothing is known about activity on intracellular L. pneumophila within their hosts, and subsequently mechanis...Continue Reading

References

Jun 15, 2000·Nature Cell Biology·M Carmo-FonsecaI Campos
Aug 15, 2000·FEBS Letters·D WadeP Kuusela
Aug 6, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Qian ChenDe Yang
Oct 30, 2004·The Journal of Biological Chemistry·Maria Luisa MangoniLuis Rivas
Mar 24, 2010·The Journal of Biological Chemistry·Feten AbbassiAli Ladram
Apr 9, 2010·Clinical Microbiology Reviews·Hayley J NewtonElizabeth L Hartland
Feb 5, 2011·Peptides·A MarchandJ M Berjeaud
Feb 16, 2011·Journal of Medicinal Chemistry·Maria Luisa MangoniPaolo Grieco
Jun 14, 2012·PloS One·Jorge AlvarUNKNOWN WHO Leishmaniasis Control Team
Oct 20, 2012·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Min-Duk SeoBong-Jin Lee
Feb 12, 2014·Antimicrobial Agents and Chemotherapy·Antonio Di GraziaMaria Luisa Mangoni
Jun 13, 2014·Journal of Peptide Science : an Official Publication of the European Peptide Society·Andres LombanaVincent Humblot
Oct 16, 2014·Biochimica Et Biophysica Acta·Yana R MusinovaEugene V Sheval
Jan 8, 2015·Frontiers in Microbiology·Peter RobertsonRafael A Garduño
Feb 11, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Gabriela A EggimannPaul W Denny
Jul 4, 2015·Current Topics in Medicinal Chemistry·Maria Luisa MangoniVincenzo Luca
Aug 19, 2015·Nucleus·Robert M MartinM Cristina Cardoso
Nov 26, 2015·Nucleic Acids Research·Guangshun WangZhe Wang
Apr 19, 2016·Nature Reviews. Immunology·Robert E W HancockErin E Gill
Apr 23, 2016·Frontiers in Bioscience (Landmark Edition)·Ali Ladram, Pierre Nicolas
May 21, 2016·Respirology : Official Journal of the Asian Pacific Society of Respirology·Heather L IsenmanDavid R Murdoch
Feb 6, 2017·Infectious Disease Clinics of North America·Abdullah Chahin, Steven M Opal
May 10, 2017·Journal of Tissue Engineering and Regenerative Medicine·Mathilde PohinJean-François Jégou
Dec 14, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jelena M PanticMiodrag L Lukic
Dec 19, 2017·Frontiers in Cellular and Infection Microbiology·David K BoamahTamara J O'Connor
Feb 7, 2018·Frontiers in Cellular and Infection Microbiology·Giulia OlivaCarmen Buchrieser
Feb 28, 2018·Antimicrobial Agents and Chemotherapy·M E MarcocciL Nencioni
Mar 15, 2018·Frontiers in Cellular and Infection Microbiology·Arwa Abu Khweek, Amal O Amer
Nov 6, 2018·Medical Microbiology and Immunology·Changle WangMei Liang

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Citations

Aug 17, 2020·International Journal of Molecular Sciences·Surajit Bhattacharjya, Suzana K Straus
Feb 23, 2021·Frontiers in Cellular and Infection Microbiology·Danieli F BucciniOctavio L Franco

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Methods Mentioned

BETA
fluorescence microscopy
transmission electron microscopy
scraping

Software Mentioned

ImageJ
GraphPad Prism

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