In vitro anti-inflammatory and anti-cancer activities of Cuscuta reflexa Roxb

Journal of Ethnopharmacology
V SureshV V Asha

Abstract

To determine anti-inflammatory and anti-cancer activities of Cuscuta reflexa in cell lines (in vitro). Anti-inflammatory activity of the water extract was analysed in vitro using lipopolysaccharide (LPS) induced inflammatory reactions in murine macrophage cell line RAW264.7. The expression of COX-2 and TNF-α genes involved in inflammation was analysed by SQ RT-PCR. EMSA was conducted to analyse the influence of the extract on NF-κB signalling. Anti-cancer activity was analysed on Hep3B cells by MTT assay, DAPI staining, annexin V staining and SQ-RT PCR analysis of BAX, Bcl-2, p53 and survivin. The extract down regulated LPS induced over expression of TNF-α and COX-2 in RAW264.7 cells; blocked NF-κB binding to its motifs and induced apoptosis in Hep3B cells as evidenced from MTT, DAPI staining and annexin V staining assays. The extract up regulated pro-apoptotic factors BAX and p53, and down regulated anti-apoptotic factors Bcl-2 and survivin. The study showed that Cuscuta reflexa inhibits LPS induced inflammatory responses in RAW264.7 cells through interplay of TNF-α, COX-2 and NF-κB signalling. It induced apoptosis in Hep3B cells through the up regulation of p53, BAX and down regulation of Bcl-2 and survivin.

References

Jan 1, 1994·Annual Review of Medicine·K J Tracey, A Cerami
Mar 5, 1999·Cellular and Molecular Life Sciences : CMLS·S Bates, K H Vousden
Mar 20, 2002·Cytokine & Growth Factor Reviews·Fran Balkwill
Dec 20, 2002·Nature·Lisa M Coussens, Zena Werb
Dec 23, 2003·Cancer Biology & Therapy·A-C GouletMark A Nelson
Aug 17, 2004·The Journal of Biological Chemistry·Mark J DemmaBimalendu Dasmahapatra
Aug 18, 2004·Biochemical Pharmacology·Shishir Shishodia, Bharat B Aggarwal
Apr 26, 2005·Journal of Ethnopharmacology·Letícia Veras Costa-LotufoManoel Odorico de Moraes
Jun 10, 2005·Nature·Alberto Mantovani
Jun 14, 2005·Journal of Ethnopharmacology·Tuzz-Ying SongGow-Chin Yen
Apr 20, 2006·Cancer Letters·N K SahP S Bisen
Aug 8, 2006·Biochemical Pharmacology·Bharat B AggarwalGautam Sethi

Citations

Mar 14, 2012·Zhong xi yi jie he xue bao = Journal of Chinese integrative medicine·Satish PatelVinod K Dixit
Jan 1, 2012·African Journal of Traditional, Complementary, and Alternative Medicines : AJTCAM·Mohammed RahmatullahRownak Jahan
Sep 22, 2015·Journal of Ethnopharmacology·Prakash Roy ChoudhuryAnupam Das Talukdar
May 10, 2012·Evidence-based Complementary and Alternative Medicine : ECAM·Yu-Ling HoGuan-Jhong Huang
Oct 15, 2013·Natural Product Research·Prajwal PaudelWilliam N Setzer
Jul 16, 2016·The Journal of Pharmacy and Pharmacology·Mohammad Hosein FarzaeiBahman Mehraban
May 18, 2018·The Journal of Pharmacy and Pharmacology·Sheena PhilipAsha V Vasumathi

Related Concepts

Birc5 protein, mouse
Anti-Inflammatory Agents
Antineoplastic Agents, Phytogenic
Plant Extracts
Repressor Molecules
Signal Transduction
Protein p53
Ig-EBP-1
Apoptosis, Intrinsic Pathway
Reverse Transcriptase Polymerase Chain Reaction

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Anti-inflammatory Treatments

A drug or substance that reduces inflammation (redness, swelling, and pain) in the body. Anti-inflammatory agents block certain substances in the body that cause inflammation and swelling. Discover the latest research on anti-inflammatory treatments here

BCL-2 Family Proteins

BLC-2 family proteins are a group that share the same homologous BH domain. They play many different roles including pro-survival signals, mitochondria-mediated apoptosis and removal or damaged cells. They are often regulated by phosphorylation, affecting their catalytic activity. Here is the latest research on BCL-2 family proteins.

Antiphospholipid Syndrome

Antiphospholipid syndrome or antiphospholipid antibody syndrome (APS or APLS), is an autoimmune, hypercoagulable state caused by the presence of antibodies directed against phospholipids.

Related Papers

Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association
R W OwenH Bartsch
Journal of Ethnopharmacology
Letícia Veras Costa-LotufoManoel Odorico de Moraes
Cell
Sergei I GrivennikovMichael Karin
© 2021 Meta ULC. All rights reserved