In vitro antibacterial activity of fluoroquinolones against Porphyromonas gingivalis strains

The Journal of Antimicrobial Chemotherapy
S EickW Pfister

Abstract

The objective of the study was to evaluate the in vitro activity of ciprofloxacin, gatifloxacin and moxifloxacin against 16 Porphyromonas gingivalis strains. MICs of the quinolones were established by Etest and the agar dilution technique. Experiments focused on determination of the spontaneous mutation rate and the induction of resistant strains, using 0.25-fold MIC of antibiotic. Fragments of gyrA and gyrB as well as of parC were sequenced. Moxifloxacin and gatifloxacin had very low MIC values. Subinhibitory concentrations of the fluoroquinolones rapidly induced mutations. The spontaneous mutation rate was strain- and quinolone-dependent; the lowest rate was encountered after moxifloxacin. The predicted serum concentrations of the quinolones were bactericidal to wild-type strains, but 100 mg/L of each tested quinolone was insufficient to kill a mutant exhibiting moderate resistance. Often the mutants exhibited high resistance to >/=32 mg/L. All these mutants bore a Ser-83-->Phe substitution in GyrA. DNA gyrase is the primary target of fluoroquinolones in P. gingivalis. In terms of the achievable level in the gingival fluid and the MICs, moxifloxacin might prevent the development of resistance and may be an alternative in the ...Continue Reading

Citations

Jan 4, 2007·Analytical and Bioanalytical Chemistry·Patrícia NevesPaula Gameiro
Sep 17, 2010·Journal of Periodontology·Thomas F FlemmigThomas Beikler
May 11, 2013·Journal of Periodontology·Georgios N Belibasakis, Thomas Thurnheer
Jun 16, 2010·Journal of Periodontal Research·C M ArdilaI C Guzmán
Nov 5, 2016·International Journal of Antimicrobial Agents·A J van WinkelhoffG J Wekema
Aug 7, 2007·Expert Review of Anti-infective Therapy·Hans-Peter Horz, Georg Conrads
Jan 21, 2017·Anaerobe·Teresa OlczakZbigniew Ciunik
Dec 24, 2017·Journal of Biomedical Materials Research. Part a·Meghan E E WrightJ Paul Santerre

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