PMID: 8971041Dec 1, 1996Paper

In vitro antigen challenge of human antibody libraries for vaccine evaluation: the human immunodeficiency virus type 1 envelope

Journal of Virology
P W ParrenDennis R Burton

Abstract

Human antibody responses, or versions thereof, can be cloned as phage display libraries. In vaccine evaluation, the possibility therefore exists of challenging the human response in vitro, rather than in vivo, in order to assist in establishing the most promising vaccine leads. The characteristics of the antibodies retrieved directly indicate the strengths and weaknesses of the vaccine at the molecular level. We applied this approach to compare recombinant and native human immunodeficiency virus type 1 envelope preparations. We conclude that recombinant gp160, gp140, and, to a lesser extent, gp120 present epitopes around the CD4 binding site in a conformation different from that of the native multimer and contrary to expected vaccine requirements. Antibodies to the potently neutralizing b12 epitope were selected preferentially from an immune library by purified human immunodeficiency virus type 1 virions. This suggests that b12 is a major epitope on the virions, in contrast to recombinant envelope preparations, in which related, weakly neutralizing epitopes predominate. Although the majority of virions in the preparation used are expected to be noninfective, it appears that they predominantly express a native envelope configura...Continue Reading

References

Oct 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·C F BarbasE Norrby
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Citations

Sep 10, 1998·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·R BurioniM Clementi
Jan 20, 1999·Proceedings of the National Academy of Sciences of the United States of America·A A McCormickR Levy
Apr 1, 1997·Nature Medicine·P W ParrenQ J Sattentau
Sep 18, 1997·Proceedings of the National Academy of Sciences of the United States of America·D R Burton
Jan 17, 2021·Microorganisms·Saman Fouladirad, Horacio Bach
Sep 1, 2000·Journal of Molecular Biology·M A PoulJ D Marks

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