PMID: 6169778Oct 1, 1981Paper

In vitro antigen-induced, antigen-specific antibody production in man. Specific and polyclonal components, kinetics, and cellular requirements

The Journal of Experimental Medicine
H C LaneA S Fauci

Abstract

A highly specific and reproducible antigen-induced, antigen-specific culture and assay system for antibody production by human peripheral blood B lymphocytes has been developed. The system is clearly T cell and monocyte dependent and is independent of exogenous mitogens. The major factors in our ability to trigger specific antibody production with antigen alone have been the use of extremely low concentrations of antigen in vitro (doses several orders of magnitude below those inducing a peak blastogenic response), careful attention to in vitro cell density and culture vessel geometry, and appreciation of the kinetics of the circulating antigen-inducible B cell repertoire. A dichotomy and overlap between antigen-induced, antigen-specific and antigen-induced, polyclonal responses was observed in the study of doubly immunized individuals. Whereas antibody responses highly specific for the antigen in culture were observed under one set of culture conditions (flat-bottomed vessels, 1.5 x 10(6) cells), switching to another culture system (round-bottomed vessels, 5 x 10(5) cells) resulted in polyclonal responses to antigen. Despite these culture condition-related differences in the induction of antibody synthesis, the suppression of s...Continue Reading

References

Sep 1, 1979·European Journal of Immunology·A A AugustinH Cosenza
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Jun 1, 1976·Scandinavian Journal of Immunology·R J Winchester, S M Fu
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Citations

Mar 1, 1985·Journal of Clinical Immunology·A G PalestineR B Nussenblatt
Jul 1, 1988·Journal of Clinical Immunology·S J AndersonA R Lawton
Dec 16, 1983·Journal of Immunological Methods·P R MacdonaldP S Crosier
May 11, 1984·Journal of Immunological Methods·A L MooreM P Hacker
Dec 17, 1985·Journal of Immunological Methods·J Lagacé, B R Brodeur
Oct 10, 1985·Journal of Immunological Methods·J M WeilerR Yarchoan
Mar 28, 1996·Journal of Immunological Methods·J A OwenZ L Jonak
Jun 1, 1988·The Journal of Allergy and Clinical Immunology·D Y LeungR S Geha
Oct 1, 1990·Toxicology Letters·R M Pillai, R R Watson
Feb 7, 2009·Nature Protocols·Jean-Charles Grivel, Leonid Margolis
Oct 25, 1984·The New England Journal of Medicine·H C LaneL C Edgar
Mar 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·M K GornyS Zolla-Pazner
Aug 1, 1982·The Journal of Experimental Medicine·D J VolkmanD M Neville
Oct 1, 1988·The Journal of Experimental Medicine·M G PetersE J Brown
Dec 24, 1997·Journal of Periodontal Research·C L HahnJ G Tew
Apr 1, 1983·The Journal of Clinical Investigation·J H Kehrl, A S Fauci
Aug 1, 1983·The Journal of Clinical Investigation·H C LaneA S Fauci
Jun 1, 1989·The Journal of Clinical Investigation·M G PetersR P MacDermott
Jun 1, 1990·The Journal of Clinical Investigation·C L KingT B Nutman
Oct 1, 1983·Journal of Clinical Immunology·E De Bernardo, T F Davies
Oct 1, 1987·Immunological Investigations·R Hong
Jul 16, 2008·Seminars in Immunology·Michael M Lederman, Leonid Margolis

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