In vitro antiplasmodial activity, cytotoxicity, and gas chromatography - flame ionization detector metabolites fingerprint of extracts and fractions from Tetrorchidium didymostemon.

Journal of Ayurveda and Integrative Medicine
Osamudiamen EbohonEhimwenma Sheena Omoregie

Abstract

Tetrorchidium didymostemon is used as an antimalarial remedy in southern Nigeria. This study was aimed at providing scientific validation for the use of T. didymostemon in the treatment of malaria in Nigeria. Plasmodium falciparum 3D7 (Pf3D7) strain was cultured and maintained in fresh O+ human erythrocytes. Standard methods were used to evaluate in vitro antiplasmodial activity, cytotoxic effect on Vero cell line, phytochemical screening, and antioxidant capacity. Gas Chromatography - Flame Ionization Detector (GC-FID) metabolite fingerprinting of the most potent fraction was carried out. The methanol leaf extract had higher antiplasmodial activity (IC50Pf3D7 = 25 ± 0.21 μg/mL) in comparison with the stem bark extract (SBE) (IC50Pf3D7 = 50 ± 0.94 μg/mL). The n-hexane fraction of the leaf extract had the best antiplasmodial activity (IC50Pf3D7 = 3.92 ± 0.46 μg/mL) and selectivity index. This was followed by the dichloromethane (IC50Pf3D7 = 12.5 ± 1.32 μg/mL), ethyl acetate (IC50Pf3D7 = 35.0 ± 4.80 μg/mL), and hydromethanol fraction which was inactive (IC50Pf3D7 > 100 μg/mL). All extracts and fractions were not toxic on Vero cell line (CC50 > 1000 μg/mL). The n-hexane and dichloromethane fractions had the highest amount of phyto...Continue Reading

References

Aug 20, 1976·Science·W Trager, J B Jensen
Jun 27, 2001·The American Journal of Tropical Medicine and Hygiene·J G Breman
Aug 23, 2001·The Journal of Infectious Diseases·T E Wellems, C V Plowe
Jan 11, 2003·Natural Product Reports·Sianne Schwikkard, Fanie R van Heerden
Jun 3, 2004·The Lancet Infectious Diseases·Simon I HayRobert W Snow
Jun 25, 2004·Antimicrobial Agents and Chemotherapy·Herbert Rodrigues GoulartAlejandro M Katzin
Apr 9, 2005·Journal of Ethnopharmacology·Guédé Noël ZirihiPhilippe Grellier
Nov 10, 2006·Drug Resistance Updates : Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy·W Sirawaraporn
Aug 21, 2007·Phytotherapy Research : PTR·E O AjaiyeobaC W Wright
Dec 10, 2008·The New England Journal of Medicine·Harald NoedlUNKNOWN Artemisinin Resistance in Cambodia 1 (ARC1) Study Consortium
Jul 31, 2009·The New England Journal of Medicine·Arjen M DondorpNicholas J White
May 26, 2011·Malaria Journal·Jane AchanUmberto D'Alessandro
Aug 29, 2013·Revista Da Sociedade Brasileira De Medicina Tropical·Nnennaya Anthony Ajayi, Kingsley Nnanna Ukwaja
Mar 13, 2014·Trends in Microbiology·Huizhi WangDavid A Scott
Oct 28, 2015·Antimicrobial Agents and Chemotherapy·Agathe BoussaroqueBruno Pradines
Dec 10, 2015·Journal of Intercultural Ethnopharmacology·Bashir LawalBlessing Uchenna Alozieuwa
Sep 22, 2016·Malaria Journal·Christopher Dean GoodmanHelle Wangensteen
Mar 23, 2017·Pharmaceutical Biology·Jude E OkokonDinkar Sahal
Mar 23, 2017·Journal of Ethnopharmacology·Olivia JansenMichel Frédérich
Feb 6, 2018·The Lancet Infectious Diseases·Roberto AmatoDominic P Kwiatkowski
Feb 7, 2018·BMC Complementary and Alternative Medicine·Nashwan Abdullah Al-AfifiAnand Ramanathan
Oct 17, 2019·Revista Da Sociedade Brasileira De Medicina Tropical·Osamudiamen EbohonEhimwenma Sheena Omoregie

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