In vitro antiproliferative and apoptosis-inducing properties of a mononuclear copper(II) complex with dppz ligand, in two genotypically different breast cancer cell lines

Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine
Rajakumar DhivyaMohammad Abdulkader Akbarsha

Abstract

In the background that there is concerted effort to discover newer metal-based cancer chemotherapeutic agents that could overcome the limitations in cisplatin and that copper, a biocompatible and redox-active metal, offers potential as alternative to cisplatin, the present study was undertaken to investigate the in vitro anti-proliferative properties of the mononuclear copper(II)complex [Cu(L)(diimine)] + where LH = 2-[(2-dimethylaminoethylimino)methyl]phenol and diimine = dipyrido[3,2-a:2',3'-c]phenazine (dppz) using breast cancer cell lines MCF-7 (ER(+ve) and p53(WT)) and MDA-MB-231(ER(-ve) and p53(mutant)) when cisplatin was used as positive control. The complex affected the viability of both the cell lines in dose-as well as duration-dependent manner as revealed in the MTT assay. The 24 and 48 h IC50 of the complex were several times lesser than those of cisplatin, and within this huge difference the efficacy of the complex was much superior with MCF-7 cell compared to MDA-MB-231 cell. The cell death was preferentially apoptosis, though necrosis also occurred to a certain extent. These inferences were substantiated by AO/EB fluorescent staining, Hoechst staining, assessment of mitochondrial transmembrane potential, comet as...Continue Reading

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Citations

Oct 27, 2018·Journal of Cellular Biochemistry·Mohamed A Abdel-MohsenMarihan A Helal
Jun 24, 2018·Applied Biochemistry and Biotechnology·Nasima Arshad, Shahid Iqbal Farooqi
Dec 4, 2019·Dalton Transactions : an International Journal of Inorganic Chemistry·Ming LiuJing-Yuan Xu
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Jun 23, 2021·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Balakrishnan GowdhamiMohammad A Akbarsha

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