In vitro antiviral activity of Lactobacillus casei and Bifidobacterium adolescentis against rotavirus infection monitored by NSP4 protein production

Journal of Applied Microbiology
N N Olaya GalánM F Gutierrez Fernandez

Abstract

The aim of this study was to determine the antiviral activity of four probiotic metabolites (Lactobacillus and Bifidobacetrium species) against rotavirus in vitro infection monitored by the NSP4 protein production and Ca(2+) release. The antiviral effect of the metabolites was performed due a comparison between a blocking model and an intracelullar model on MA104 cells, with the response of NSP4 production and Ca(2+) liberation measured by flow cytometry. Significant results were obtained with the metabolites of Lactobacillus casei, and Bifidobacterium adolescentis in the reduction of the protein production (P = 0·04 and P = 0·014) and Ca(2+) liberation (P = 0·094 and P = 0·020) in the intracellular model, which suggests a successful antiviral activity against RV infection. This study demonstrates that probiotic metabolites were able to interfere with the final amount of intracellular NSP4 protein and a successful Ca(2+) regulation, which suggests a new approach to the mechanism exerted by probiotics against the rotavirus infection. A novel anti-rotaviral effect exerted by probiotic metabolites monitored by the NSP4 protein during the RV in vitro infection and the effect on the Ca(2+) release is reported; suggesting a reduction...Continue Reading

References

Jun 5, 1998·Trends in Pharmacological Sciences·M TreimanS B Christensen
Mar 29, 2001·Journal of the American Dietetic Association·L Kopp-Hoolihan
May 30, 2002·Virology·Victoria PandoSusana López
Mar 23, 2004·Anales de pediatría : publicación oficial de la Asociación Española de Pediatría (A.E.P.)·E Román RiechmannA Sánchez-Fauquier
Dec 15, 2004·Journal of Virology·Tomas LópezSusana López
Apr 2, 2005·Viral Immunology·Judith M BallRebecca D Parr
Jan 31, 2007·International Journal of Food Microbiology·Tanja BotićAvrelija Cencic
Nov 21, 2007·Microbes and Infection·Florence Colbère-GarapinAriane van de Moer
May 22, 2009·Gastroenterology·Harry B Greenberg, Mary K Estes
Sep 29, 2009·International Journal of Medical Microbiology : IJMM·Tobias A Oelschlaeger
Jan 2, 2010·American Journal of Physiology. Gastrointestinal and Liver Physiology·Geetu RahejaPradeep K Dudeja
Jan 29, 2010·International Journal of Food Microbiology·Petros A MaragkoudakisAvrelija Cencic
Apr 24, 2010·Current Opinion in Biotechnology·Seppo SalminenHani El-Nezami
Dec 1, 2010·Pediatrics·Dan W ThomasUNKNOWN American Academy of Pediatrics Section on Gastroenterology, Hepatology, and Nutrition
Jul 19, 2011·International Journal of Food Microbiology·D A RussellC Stanton
Oct 14, 2011·Journal of Clinical Gastroenterology·Stefano Guandalini
Oct 18, 2011·Applied and Environmental Microbiology·José Antonio Moreno MuñozMontserrat Rivero
Jan 24, 2012·Nature Reviews. Microbiology·Shane D TraskJohn T Patton
Jul 11, 2012·Journal of Tropical Medicine·Ozlem ErdoğanFaruk Oktem
Oct 6, 2012·Annals of Nutrition & Metabolism·Miriam Bermudez-BritoAngel Gil
Oct 12, 2012·Archives of Pharmacal Research·Hyang Mi AnNam Joo Ha
Oct 13, 2012·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·I-Chiao LeePeter A Bron
Nov 10, 2012·World Journal of Gastroenterology : WJG·Hanna VentolaReetta Holma
Jan 29, 2013·Daru : Journal of Faculty of Pharmacy, Tehran University of Medical Sciences·Rezvan ZabihollahiMohammad Hossein Modarressi
Mar 26, 2013·Current Infectious Disease Reports·Penelope H Dennehy
May 3, 2013·Probiotics and Antimicrobial Proteins·Nicolás I TorresMichael L Chikindas
May 10, 2013·Archives of Pharmacal Research·Do Kyung LeeNam Joo Ha
Apr 24, 2014·Human Vaccines & Immunotherapeutics·Catherine YenUmesh D Parashar
Mar 6, 2015·Frontiers in Microbiology·Konstantinos PapadimitriouEffie Tsakalidou
Apr 30, 2015·Disease-a-month : DM·Barry A Mizock
May 29, 2015·Biotechnology, Biotechnological Equipment·Min Ji KimNam Joo Ha
Jul 17, 2015·The Nurse Practitioner·Amita Avadhani, Lorraine Steefel

❮ Previous
Next ❯

Citations

Jun 11, 2020·Critical Reviews in Food Science and Nutrition·Jagrani MinjRakesh Kumar Sharma
Apr 17, 2018·Veterinary World·Amr M AbdouM A Bakry
Sep 26, 2020·Frontiers in Microbiology·Santosh Kumar TiwariMichael L Chikindas
Jun 22, 2017·Archives of Microbiology·Guadalupe Gonzalez-OchoaPatricia Tamez-Guerra
Apr 23, 2017·Probiotics and Antimicrobial Proteins·Karem Prunella Fernandez-DuarteMaria Fernanda Gutierrez-Fernandez
Jul 15, 2021·BioMed Research International·Irina V SolovevaRinat A Maksyutov

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antivirals

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.

Antivirals (ASM)

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.