In vitro antiviral activity of dehydroepiandrosterone, 17 synthetic analogs and ERK modulators against herpes simplex virus type 1

Antiviral Research
Nicolás I TorresMónica B Wachsman

Abstract

In the present study the in vitro antiviral activity of dehydroepiandrosterone (DHEA) and 17 synthetic derivatives against herpes simplex type 1 (HSV-1) was determined. DHEA, epiandrosterone (EA), two synthetic DHEA analogs and three synthetic EA analogs showed a selective inhibitory effect on HSV in vitro multiplication. DHEA and E2, a synthetic derivative of EA, were not found to be virucidal to cell-free HSV-1 and did not impair virus adsorption or penetration. We determined that treatment with both compounds decreased viral protein synthesis. Moreover, inhibitory effect of DHEA and E2 on extracellular viral titer was stronger than the inhibition found on total viral infectivity, suggesting that the antiherpetic activity of these compounds may also be in part due to an inhibition in virus formation and release. Since DHEA is a known Raf/MEK/ERK signaling pathway activator, we studied the role of this pathway on HSV-1 infection. ERK1/2 phosphorylation was stimulated in HSV-1 infected cultures. UO126, a Raf/MEK/ERK signaling pathway inhibitor, impaired viral multiplication, while anisomycin, an activator of this pathway, enhanced it. Treatment with DHEA 6 h before infection enhanced HSV-1 multiplication. On the contrary, pre-t...Continue Reading

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Citations

Jan 15, 2014·The Journal of General Virology·María Eugenia RodríguezViviana Castilla
Dec 22, 2014·Medical Microbiology and Immunology·Carlos Alberto BuenoLaura Edith Alché
May 4, 2017·The Journal of Organic Chemistry·David TejedorFernando García-Tellado
May 18, 2016·Chemistry : a European Journal·K C NicolaouAbdelatif ElMarrouni
Dec 2, 2018·Future Medicinal Chemistry·Shaoyong KeYanhong Wei
Mar 21, 2020·PLoS Neglected Tropical Diseases·Gretchen EhrenkauferUpinder Singh
Nov 20, 2016·Folia Microbiologica·Daniele Zendrini RechenchoskiCarlos Nozawa

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