In vitro assembly of a complete, pentaglycine interpeptide bridge containing cell wall precursor (lipid II-Gly5) of Staphylococcus aureus

Molecular Microbiology
Tanja SchneiderImke Wiedemann

Abstract

Staphylococcus aureus peptidoglycan is cross-linked via a characteristic pentaglycine interpeptide bridge. Genetic analysis had identified three peptidyltransferases, FemA, FemB and FemX, to catalyse the formation of the interpeptide bridge, using glycyl t-RNA as Gly donor. To analyse the pentaglycine bridge formation in vitro, we purified the potential substrates for FemA, FemB and FemX, UDP-MurNAc-pentapeptide, lipid I and lipid II and the staphylococcal t-RNA pool, as well as His-tagged Gly-tRNA-synthetase and His-tagged FemA, FemB and FemX. We found that FemX used lipid II exclusively as acceptor for the first Gly residue. Addition of Gly 2,3 and of Gly 4,5 was catalysed by FemA and FemB, respectively, and both enzymes were specific for lipid II-Gly1 and lipid II-Gly3 as acceptors. None of the FemABX enzymes required the presence of one or two of the other Fem proteins for activity; rather, bridge formation was delayed in the in vitro system when all three enzymes were present. The in vitro assembly system described here will enable detailed analysis of late, membrane-associated steps of S. aureus peptidoglycan biosynthesis.

References

Mar 1, 1990·Antimicrobial Agents and Chemotherapy·E A Somner, P E Reynolds
Oct 1, 1985·Analytical Biochemistry·P K SmithD C Klenk
Aug 1, 1993·Journal of Bacteriology·C J Green, B S Vold
Apr 29, 1998·Antimicrobial Agents and Chemotherapy·B Ling, B Berger-Bächi
Aug 4, 1999·Proceedings of the National Academy of Sciences of the United States of America·S RohrerB Berger-Bächi
May 11, 2000·Proceedings of the National Academy of Sciences of the United States of America·J van Heijenoort, L Gutmann
Aug 15, 2002·Structure·Timothy E BensonRobert L Garlick
Sep 27, 2002·The Journal of Biological Chemistry·Ahmed BouhssMichel Arthur
Mar 29, 2003·The Journal of Biological Chemistry·Eefjan BreukinkBen de Kruijff

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Citations

Nov 15, 2008·The Journal of Membrane Biology·Katrin ChristGerd Bendas
Jun 6, 2007·Bioorganic & Medicinal Chemistry Letters·Elena CressinaTimothy D H Bugg
Jun 8, 2010·Journal of Medicinal Chemistry·Jed F Fisher, Shahriar Mobashery
Oct 25, 2012·The Journal of Biological Chemistry·Jesper S OeemigReinhard Wimmer
Mar 22, 2012·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Hélène BarreteauDominique Mengin-Lecreulx
Mar 22, 2012·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Anna MüllerTanja Schneider
Feb 17, 2010·The Journal of Antimicrobial Chemotherapy·Stamatina GiannouliAthanassios Tsakris
Oct 13, 2007·Nucleic Acids Research·Régis VilletMichel Arthur
Jan 20, 2009·Nucleic Acids Research·Matthieu FonvielleMichel Arthur
Nov 9, 2012·Molecular Oral Microbiology·D OsbourneH M Fletcher
May 29, 2010·Science·Tanja SchneiderHans-Henrik Kristensen
Apr 6, 2011·Antimicrobial Agents and Chemotherapy·E RubinchikR E W Hancock
Jun 2, 2011·Antimicrobial Agents and Chemotherapy·Hideki HashizumeAkio Nomoto
Nov 26, 2009·Antimicrobial Agents and Chemotherapy·Kenji MiuraHirokazu Arimoto
Mar 30, 2006·Antimicrobial Agents and Chemotherapy·Raquel Regina BonelliImke Wiedemann
Jun 10, 2008·Applied and Environmental Microbiology·Beatriz MartínezImke Wiedemann
May 12, 2009·Applied and Environmental Microbiology·T BöttigerI Wiedemann
Mar 14, 2007·Applied and Environmental Microbiology·Mariella RivasDavid S Holmes
Apr 7, 2006·Applied and Environmental Microbiology·Imke WiedemannBeatriz Martínez
Apr 14, 2010·Infection and Immunity·Vera SassHans-Georg Sahl
Aug 23, 2006·Journal of Bacteriology·Angelika GründlingOlaf Schneewind
Mar 4, 2005·Journal of Bacteriology·Dianah BarrettSuzanne Walker
Mar 21, 2006·Journal of Bacteriology·Angelika Gründling, Olaf Schneewind
Dec 8, 2007·Microbiology and Molecular Biology Reviews : MMBR·Jean van Heijenoort
Sep 6, 2007·BMC Genomics·Judith HübscherBrigitte Berger-Bächi
Jan 5, 2012·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Hiroshi Tomoda
Apr 8, 2014·Proceedings of the National Academy of Sciences of the United States of America·Michaela WenzelJulia Elisabeth Bandow
Jan 29, 2016·Biochemistry·Lorenza FavrotOlivia Vergnolle
Oct 12, 2010·Biophysical Chemistry·Saad Al-KaddahGerd Bendas
Nov 19, 2008·Biochimie·Stamatina GiannouliConstantinos Stathopoulos
Apr 8, 2015·The FEBS Journal·William J BradshawK Ravi Acharya
Mar 11, 2016·Scientific Reports·Yasuhiko MatsumotoKazuhisa Sekimizu
Jan 21, 2012·Wiley Interdisciplinary Reviews. RNA·Kiley Dare, Michael Ibba

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