PMID: 9448274Mar 14, 1998Paper

In vitro assembly of a ribonucleoprotein particle corresponding to the platform domain of the 30S ribosomal subunit

Proceedings of the National Academy of Sciences of the United States of America
S C AgalarovA S Spirin

Abstract

A fragment of the 16S RNA of Thermus thermophilus corresponding to the central domain (nucleotides 547-895) has been prepared by transcription in vitro. Incubation of this fragment with the total 30S ribosomal proteins has resulted in the formation of a compact 12S ribonucleoprotein particle. This particle contained five T. thermophilus proteins corresponding to Escherichia coli ribosomal proteins S6, S8, S11, S15, and possibly S18, all of which were previously shown to interact with the central domain of the 16S RNA and to be localized in the platform (side bulge) of the 30S ribosomal subunit. When examined by electron microscopy, isolated particles have an appearance that is similar in size and shape to the corresponding morphological features of the 30S subunit. We conclude that the central domain of the 16S RNA can independently and specifically assemble with a defined subset of ribosomal proteins into a compact ribonucleoprotein particle corresponding to the platform (side bulge) of the 30S subunit.

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Citations

Jan 20, 2004·Journal of Molecular Biology·Indu Jagannathan, Gloria M Culver
Nov 3, 2004·Nature Reviews. Molecular Cell Biology·Renée SchroederKatharina Semrad
May 24, 2003·Cold Spring Harbor Symposia on Quantitative Biology·M I Recht, J R Williamson
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May 4, 2005·Molecular Cell·Manjuli R SharmaRajendra K Agrawal
Jun 15, 1999·Journal of Molecular Biology·L Holmberg, H F Noller
Feb 1, 2017·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Joseph H Davis, James R Williamson
Jan 28, 2003·Biopolymers·Gloria M Culver
Mar 5, 2021·Proceedings of the National Academy of Sciences of the United States of America·Naoto HoriD Thirumalai
Jul 28, 2020·Journal of Molecular Biology·Michelle R GibbsKurt Fredrick
Oct 17, 2001·Journal of Molecular Biology·M I Recht, J R Williamson
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Sep 1, 2008·EcoSal Plus·Gloria M Culver, Narayanaswamy Kirthi
Jun 13, 2021·Cellular and Molecular Life Sciences : CMLS·Sergey V Razin, Alexey A Gavrilov

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