PMID: 8464918Apr 1, 1993Paper

In vitro assembly of multiprotein complexes containing alpha, beta, and gamma tubulin, heat shock protein HSP70, and elongation factor 1 alpha

Proceedings of the National Academy of Sciences of the United States of America
V T Marchesi, N Ngo

Abstract

We have isolated two sets of multiprotein complexes from supernatants from high-speed centrifugation of nocodazole-arrested CHO cells. One set, assembled in vitro after a 37 degrees C incubation in the presence of ATP or GTP, is composed of equivalent amounts of alpha- and beta-tubulin and a 50-kDa protein, provisionally identified as elongation factor 1 alpha. These complexes, which are heterogeneous in size when analyzed by sucrose gradient ultracentrifugation, also contain the cognate form of heat shock protein HSP70 and gamma-tubulin, a tubulin isoform of low abundance, along with other proteins known to be involved in the regulation of mitosis. Similar but distinct complexes assemble in vitro if the same extracts are incubated at 37 degrees C without added nucleotides; multiprotein complexes generated under these conditions lack HSP70 but contain instead a 43-kDa protein identified as an actin isoform. Both sets of assembled complexes exhibit a globular substructure when analyzed by electron microscopy, and their size distribution suggests that they assemble by the step-wise addition of smaller precursors. The properties of these multiprotein complexes and their presence in cells arrested in a stage between prophase and me...Continue Reading

References

Jun 1, 1979·The Journal of Cell Biology·B R Telzer, J L Rosenbaum
Sep 17, 1992·Nature·S W Clark, D I Meyer
Mar 1, 1992·The Journal of Cell Biology·C H YangM Snyder
Jun 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·K M FlahertyK C Holmes
Feb 1, 1991·The Journal of Cell Biology·A ToussonM M Valdivia
Nov 15, 1984·Nature·T Mitchison, M Kirschner

❮ Previous
Next ❯

Citations

Dec 1, 1996·Molecular Reproduction and Development·K W Wolf, H C Joshi
Jan 1, 1995·Cell Motility and the Cytoskeleton·B LiuB A Palevitz
Jan 1, 1995·Cell Motility and the Cytoskeleton·T Meads, T A Schroer
Feb 1, 1995·Current Opinion in Cell Biology·E Mandelkow, E M Mandelkow
Sep 12, 2000·European Journal of Pharmacology·S ValléeS Champion
Jan 24, 1998·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·T Wang, C A Lessman
Oct 27, 1998·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·L A WoodJ H Youson
Apr 27, 1999·The International Journal of Biochemistry & Cell Biology·R V GopalkrishnanP B Fisher
May 10, 1994·Proceedings of the National Academy of Sciences of the United States of America·N ShikamaC Brack
Jul 18, 1995·Proceedings of the National Academy of Sciences of the United States of America·R ShenP B Fisher
Jul 18, 1995·Proceedings of the National Academy of Sciences of the United States of America·S D Lidofsky
Jul 18, 1995·Proceedings of the National Academy of Sciences of the United States of America·C L BokrosL C Morejohn
Nov 1, 1993·The Journal of Cell Biology·L M TraubS Kornfeld
Oct 1, 1995·The Journal of Cell Biology·Q Li, H C Joshi
Dec 17, 2002·Cell Stress & Chaperones·Olga A IsaenkoMartin E Feder
Sep 24, 2011·Genetics·Carolyn D SilflowPaul A Lefebvre
Mar 15, 2001·Proceedings of the National Academy of Sciences of the United States of America·C Reynet, C R Kahn
May 10, 2005·Experimental Cell Research·Anjali M TeckchandaniAlexander Y Tsygankov
Nov 6, 2007·Biochimica Et Biophysica Acta·Ying-Fang YangTzu-Ling Tseng
Aug 20, 2002·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Guillermo BodegaBenjamín Fernández
Jan 24, 2007·Proteins·Gopa MitraBhabatarak Bhattacharyya
Dec 11, 1999·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·C Rasmussen, C Wiebe
Aug 30, 2020·The FEBS Journal·Maria Agnese Della-FaziaGiuseppe Servillo
May 23, 2002·European Journal of Cell Biology·Christine Daunderer, Ralph O Gräf

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.