PMID: 3046121Sep 1, 1988Paper

In vitro assembly of the outer shell of bacteriophage phi 6 nucleocapsid

N T KtistakisD Lang


Following dissociation of bacteriophage phi 6 nucleocapsid (NC) by EDTA, a particle composed of protein P8 and corresponding to the outer shell of the NC was assembled in vitro in the presence of Ca2+ and Mg2+. Assembly was obtained from soluble protein constituents above 100 micrograms/ml and was optimal within a temperature range of 22-30 degrees. Assembly did not require the presence of genomic RNA. Crosslinking results of intact NCs and in vitro-assembled outer shells suggested that protein P8 dimers are the structural subunits of the shell. Analysis of the assembly kinetics by electron microscopy suggested that ring-like particles of uniform size, packed in flat hexagonal arrays, are intermediates in outer shell assembly.


Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Aug 1, 1979·Virology·J F Lehman, L Mindich
Sep 12, 1986·Cell·D M SalunkeR L Garcea
Oct 20, 1986·Journal of Molecular Biology·P K SorgerS C Harrison
Aug 25, 1973·Journal of Molecular Biology·J S SemancikJ L Van Etten
Jun 1, 1980·Virology·L Mindich, R Davidoff-Abelson

❮ Previous
Next ❯


Dec 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·V M OlkkonenL Mindich
Jul 16, 1997·The EMBO Journal·S J ButcherS D Fuller
Feb 7, 2001·Journal of Virology·T L Tellinghuisen, R J Kuhn
Sep 19, 2014·Biochemical Society Transactions·Nicholas T KtistakisMaria Manifava
Jun 22, 2010·Phytotherapy Research : PTR·Odilon Djakpo, Weirong Yao
Jun 27, 1998·Journal of Molecular Biology·J T JuutiG J Thomas

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacteriophage: Phage Therapy

Phage therapy uses bacterial viruses (bacteriophages) to treat bacterial infections and is widely being recognized as an alternative to antibiotics. Here is the latest research.