PMID: 3046121Sep 1, 1988Paper

In vitro assembly of the outer shell of bacteriophage phi 6 nucleocapsid

Virology
N T KtistakisD Lang

Abstract

Following dissociation of bacteriophage phi 6 nucleocapsid (NC) by EDTA, a particle composed of protein P8 and corresponding to the outer shell of the NC was assembled in vitro in the presence of Ca2+ and Mg2+. Assembly was obtained from soluble protein constituents above 100 micrograms/ml and was optimal within a temperature range of 22-30 degrees. Assembly did not require the presence of genomic RNA. Crosslinking results of intact NCs and in vitro-assembled outer shells suggested that protein P8 dimers are the structural subunits of the shell. Analysis of the assembly kinetics by electron microscopy suggested that ring-like particles of uniform size, packed in flat hexagonal arrays, are intermediates in outer shell assembly.

References

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Citations

Dec 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·V M OlkkonenL Mindich
Jul 16, 1997·The EMBO Journal·S J ButcherS D Fuller
Feb 7, 2001·Journal of Virology·T L Tellinghuisen, R J Kuhn
Sep 19, 2014·Biochemical Society Transactions·Nicholas T KtistakisMaria Manifava
Jun 22, 2010·Phytotherapy Research : PTR·Odilon Djakpo, Weirong Yao
Jun 27, 1998·Journal of Molecular Biology·J T JuutiG J Thomas

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