In Vitro Assessment of Early Bacterial Activity on Micro/Nanostructured Ti6Al4V Surfaces

Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry
Benjamín Valdez-SalasLidia Vargas-Osuna

Abstract

It is imperative to understand and systematically compare the initial interactions between bacteria genre and surface properties. Thus, we fabricated a flat, anodized with 80 nm TiO₂ nanotubes (NTs), and a rough Ti6Al4V surface. The materials were characterized using field-emission scanning electron microscopy (FE-SEM), energy dispersive X-ray spectroscopy (EDX) and atomic force microscopy (AFM). We cultured in vitroStaphylococcus epidermidis(S. epidermidis) andPseudomonas aeruginosa(P. aeruginosa) to evaluate the bacterial-surface behavior by FE-SEM and viability calculation. In addition, the initial effects of human osteoblasts were tested on the materials. Gram-negative bacteria showed promoted adherence and viability over the flat and rough surface, while NTs displayed opposite activity with altered morphology. Gram-positive bacteria illustrated similar cellular architecture over the surfaces but with promoted surface adhesion bonds on the flat alloy. Rough surfaces supportedS. epidermidisviability, whilst NTs exhibited lower vitality. NTs advocated promoted better osteoblast organization with enhanced vitality. Gram-positive bacteria suggested preferred adhesion capability over flat and carbon-rich surfaces. Gram-negative ...Continue Reading

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Citations

Mar 7, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Krasimir VasilevPeter Zilm
Nov 23, 2019·EFORT Open Reviews·Donald J DavidsonAlexander D Liddle
Apr 22, 2018·Dental Materials : Official Publication of the Academy of Dental Materials·Heloisa N PantarotoValentim A R Barão

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Methods Mentioned

BETA
ICE
Atomic Force Microscopy
AFM
Fluorescence

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