In vitro assessment of human endothelial cell permeability: effects of inflammatory cytokines and dengue virus infection

Journal of Virological Methods
Beti Ernawati DewiIchiro Kurane

Abstract

Electrical resistance across human umbilical vein endothelial cells (HUVECs) was measured using an electrical cell sensor system. The transendothelial electrical resistance (TEER) value was used to estimate the permeability through endothelial cells in vitro. Decrease in the TEER value was associated with increase in the passage of albumin through endothelial cells in the albumin permeability assay. The effects of cytokines and dengue virus infection on the permeability of HUVECs were examined by measuring the TEER value. Tumor necrosis factor alpha (TNF-alpha) at 1 and 0.1 microg/ml decreased the TEER value, but TNF-alpha at lower dose did not. Interferon-gamma (IFN-gamma) at 1 microg/ml also decreased the TEER value. In contrast, interleukin-2 (IL-2), interleukin-6 (IL-6), interleukin-8 (IL-8), interleukin-10 (IL-10) or interferon-beta (IFN-beta) did not decrease the TEER value. The decrease in the TEER value was associated with the morphological changes of HUVECs. Dengue virus infection at a multiplicities of infection (m.o.i.) of 5 pfu/cell decreased the TEER value. Infection at an m.o.i. of 0.5 pfu/cell did not decrease the TEER value; however, addition of 0.01 microg/ml of TNF-alpha to these infected endothelial cells dec...Continue Reading

References

May 1, 1992·Archives of Biochemistry and Biophysics·J A RoyallB A Freeman
Jul 1, 1992·American Journal of Respiratory Cell and Molecular Biology·G H DownieM Friedman
Aug 15, 1989·Biochemical and Biophysical Research Communications·K ShinjoH Kawaharada
Jul 1, 1995·The Journal of Trauma·W L BifflA Banerjee
Oct 1, 1994·Experimental Cell Research·V A PolunovskyP B Bitterman
Feb 1, 1993·Journal of Virological Methods·H Killen, M A O'Sullivan
Jan 1, 1996·Journal of Neuroimmunology·H E de VriesJ Kuiper
Mar 1, 1996·Arteriosclerosis, Thrombosis, and Vascular Biology·M J RabietE Dejana
Jan 1, 1996·Microbiology and Immunology·D HoberP Wattré
Mar 14, 1998·The Journal of Infectious Diseases·D B BethellD Kwiatkowski
Jun 17, 1998·Journal of Virological Methods·S M Bonner, M A O'Sullivan
Aug 15, 1998·Annals of the New York Academy of Sciences·A MantovaniM Introna
Sep 29, 1999·Journal of Medical Virology·S GreenF A Ennis
Feb 22, 2002·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Ken-Ichiro YamadaIchiro Kurane
May 22, 2002·The Journal of Surgical Research·Tina R DesaiBruce L Gewertz
Jun 29, 2002·Journal of Virological Methods·Michael Jacobs, Michael Levin
Sep 17, 2002·American Journal of Respiratory and Critical Care Medicine·Qin WangClaire M Doerschuk
Oct 22, 2002·American Journal of Physiology. Cell Physiology·Makoto SasakiJ S Alexander
Jan 14, 2003·Journal of Applied Physiology·Masayuki HanaokaKeishi Kubo
Jun 6, 2003·American Journal of Physiology. Cell Physiology·Nilesh M Dagia, Douglas J Goetz
Jun 7, 2003·Endothelium : Journal of Endothelial Cell Research·C De StaerckeW C Hooper
Jun 10, 2003·Journal of Medical Virology·Ying-Huey HuangTrai-Ming Yeh

❮ Previous
Next ❯

Citations

Oct 14, 2009·Clinical Microbiology Reviews·Byron E E MartinaAlbert D M E Osterhaus
Nov 12, 2010·Journal of Virology·Michael G BrownRobert Anderson
Sep 16, 2011·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Aaron J CarmanMargaret S Bynoe
Apr 14, 2012·Tropical Medicine and Health·Lyre Anni Espada-Murao, Kouichi Morita
Nov 15, 2012·The American Journal of Tropical Medicine and Hygiene·Chinmay PatkarDaniel H Libraty
Mar 22, 2014·Japanese Journal of Infectious Diseases·Muhareva RaekiansyahKouichi Morita
Nov 29, 2013·Journal of Periodontology·Mizuki Tsubokawa, Soh Sato
Jan 5, 2014·Toxicological Sciences : an Official Journal of the Society of Toxicology·Stefan KustermannAdrian Roth
Feb 18, 2010·Future Virology·Rebeca Rico-Hesse
Dec 19, 2007·Seminars in Diagnostic Pathology·Anthony S-Y LeongPongsak Wannakrairot
May 22, 2007·American Journal of Surgery·David A EdelmanChristopher P Steffes
Sep 15, 2015·Lab on a Chip·Max I BogoradPeter C Searson
Jan 13, 2009·Cellular Microbiology·Yi-Ting TsaiChuan-Liang Kao
Nov 29, 2014·Reviews in Medical Virology·Peter VervaekeSandra Liekens
Aug 26, 2006·The Journal of Surgical Research·David A EdelmanChristopher Steffes
Dec 19, 2007·Drug Discovery Today. Disease Models·Dennis A Bente, Rebeca Rico-Hesse
Sep 4, 2015·Fluids and Barriers of the CNS·Margaret S BynoeDo-Geun Kim
Apr 23, 2015·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Julie K CalvertJillian M Carr
Jul 12, 2013·Virulence·Christina F Spiropoulou, Anon Srikiatkhachorn
Jul 16, 2014·Antiviral Research·Anon Srikiatkhachorn, James F Kelley
Jun 23, 2011·Acta Tropica·Alma Rosa García-TrejoCelso Ramos
Jun 5, 2008·FEMS Immunology and Medical Microbiology·Atanu Basu, Umesh C Chaturvedi
Aug 23, 2016·Journal of Autoimmunity·Carlo SelmiNoel R Rose
Sep 2, 2016·Japanese Journal of Infectious Diseases·Sivaporn InyooSa-Nga Pattanakitsakul
Dec 16, 2006·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Helena PodlovniRina Meidan
Oct 6, 2016·Scientific Reports·Misako ShigematsuTakehiko Yokomizo

❮ Previous
Next ❯

Related Concepts

Related Feeds

Arterial-Venous in Development & Disease

Arterial-venous development may play a crucial role in cardiovascular diseases. Here is the latest research.