In vitro assessment of the factors that determine the activity of the rumen microbiota for further applications as inoculum

Journal of the Science of Food and Agriculture
Alejandro BelancheDavid R Yáñez-Ruiz

Abstract

The rumen microbiota has been used as inoculum for in vitro studies and as a probiotic to improve productivity in young animals. However, great variability across studies has been noted depending on the inoculum considered. The present study aims to assess the relevance of different factors (microbial fraction, collection time, donor animal diet, fermentation substrate and inoculum preservation method) to maximize the rumen inoculum activity and set the standards for further in vitro and in vivo applications. Rumen inoculum sampled at 3 h after feeding led to greater microbial growth and activity [+12% volatile fatty acid (VFA), +17% ammonia] compared to before feeding. Similar results were noted when rumen liquid or rumen content were used as inocula. Rumen inoculum adapted to concentrate diets increased microbial activity (+19% VFA) independently of the substrate used in vitro. Freezing-thawing the inoculum, in comparison to fresh inoculum, decreased microbial activity (-14% VFA, -96% ammonia), anaerobic fungi and protozoa, with holotrichs protozoa being particularly vulnerable. Inoculum lyophilization had a stronger negative effect on microbial activity (-51% VFA) and delayed re-activation of the microbes, leading to lower l...Continue Reading

References

Jun 1, 1975·The Journal of Applied Bacteriology·B A PhillipsM E Sharpe
Aug 1, 1982·Applied and Environmental Microbiology·R M Teather
Feb 1, 1980·Applied and Environmental Microbiology·B A Dehority, J A Grubb
Feb 1, 1993·Journal of Dairy Science·A H Gustafsson, D L Palmquist
Sep 21, 2010·Applied and Environmental Microbiology·S C FernandoU Desilva
Jul 26, 2012·Journal of Animal Science·A BelancheC J Newbold
Dec 31, 2014·Applied and Environmental Microbiology·Bethany L DentonTimothy J Hackmann
Dec 5, 2015·Frontiers in Microbiology·Charles J NewboldNeil R McEwan

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Citations

May 6, 2020·Animals : an Open Access Journal From MDPI·Sonia TassonePier Giorgio Peiretti
Dec 19, 2019·Frontiers in Microbiology·Thomas HartingerKarl-Heinz Südekum
Jan 29, 2021·Frontiers in Microbiology·Alejandro BelancheDavid R Yáñez-Ruiz

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