In vitro behaviour of adult mesenchymal stem cells seeded on a bioactive glass ceramic in the SiO(2)-CaO-P(2)O(5) system

Acta Biomaterialia
L Meseguer-OlmoC L Meseguer-Ortiz

Abstract

This work describes the evaluation of a glass ceramic (55S41C4P-1300) as a potential substrate for bone tissue engineering. For that purpose, the capacity of mesenchymal stem cells (MSCs), isolated from rabbit bone marrow, to adhere, proliferate and differentiate into osteoblast (OBs) with or without 55S41C4P-1300 was investigated. Two types of culture medium, i.e. growth medium (GM) and osteogenic medium (OM), were evaluated. The bioactive 55S41C4P-1300, containing pseudowollastonite, wollastonite, tricalcium phosphate and crystoballite as crystalline phases, was obtained by heat treatment of a sol-gel glass (55SiO(2), 41CaO, 4P(2)O(5) (mol.%)) at 1300 degrees C. The results showed that the MSCs adhered, spread, proliferated and produced mineralized extracellular matrix on 55S41C4P-1300 regardless of the culture medium used. As the same time, they showed an osteoblastic phenotype, and this phenomenon was accompanied by the gradual diminution of the marker CD90 expression. The 55S41C4P-1300 was able to induce the differentiation of MSCs into OBs in the same way as OM without glass ceramic. This effect increased with the combination of 55S41C4P-1300 with OM. The glass ceramic evaluated in this work is bioactive, cytocompatible a...Continue Reading

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Citations

Apr 10, 2010·Journal of Materials Science. Materials in Medicine·Xueran Liu, Ahmed Ei-Ghannam
May 28, 2010·Tissue Engineering. Part B, Reviews·Kyobum KimJohn P Fisher
Nov 7, 2012·Tissue Engineering. Part B, Reviews·Jeong-Hui ParkIvan B Wall
Feb 11, 2014·Drug Discovery Today·Yu Long HanFeng Xu
Dec 29, 2015·Journal of Biomedical Materials Research. Part a·Maziar Montazerian, Edgar Dutra Zanotto
Feb 16, 2010·Acta Biomaterialia·Daniel Arcos, María Vallet-Regí
Aug 6, 2013·Materials Science & Engineering. C, Materials for Biological Applications·Piedad N De AzaLuis Meseguer-Olmo
May 12, 2009·Acta Biomaterialia·M Magallanes-PerdomoA H De Aza
Jun 3, 2017·Biomedical Materials·Emanuela Prado FerrazAdalberto Luiz Rosa

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