PMID: 9191975Feb 1, 1997Paper

In vitro binding of vitamin D receptor occupied by 24R,25-dihydroxyvitamin D3 to vitamin D responsive element of human osteocalcin gene

The Journal of Steroid Biochemistry and Molecular Biology
M UchidaJ W Pike

Abstract

We previously reported that 24R,25-dihydroxyvitamin D3 [24R,25(OH)2D3] activates the human osteocalcin gene (hOC) through vitamin D receptor (VDR) and vitamin D responsive element (VDRE) in the same manner as 1 alpha,25-dihydroxyvitamin D3 [1 alpha,25(OH)2 D3] [17]. In the present study, the interaction of 24R,25(OH)2D3-liganded VDR [24R,25(OH)2D3-VDR] with the hOC VDRE in vitro was investigated. The electrophoretic mobility shift assay (EMSA) revealed that the binding of 24R,25(OH)2D3-liganded VDR to the hOC VDRE was weak, even at concentrations of 24R,25(OH)2D3 10(5)-fold higher than 1 alpha,25(OH)2D3. The effect of the nuclear accessory factor (NAF), which is required for the high affinity interaction of the VDR to the VDRE, on the binding of the 24R,25(OH)2D3-VDR to the VDRE was studied using hOC VDRE affinity column chromatographic assays. In the absence of NAF, the 24R,25(OH)2D3-VDR associated weakly with the VDRE compared to the 1 alpha,25(OH)2D3-liganded VDR [1 alpha,25(OH)2D3-VDR], whereas the NAF enhanced the binding of the 24R,25(OH)2D3-VDR for the VDRE. In the absence of the hOC VDRE, the binding affinity of the 24R,25(OH)2D3-VDR for the NAF was weaker than that of 1 alpha,25(OH)2D3-VDR. These results suggest that t...Continue Reading

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Citations

Feb 17, 2001·Steroids·J P van LeeuwenH A Pols
Feb 24, 2001·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·M UchidaH Kawaguchi
Apr 14, 2015·Bone·Martin MontecinoRodrigo Aguilar
Nov 12, 2013·International Journal of Cancer. Journal International Du Cancer·Alison M MondulDemetrius Albanes
Oct 25, 2011·Journal of Neuroimmunology·Andreas LossiusTrygve Holmøy
May 29, 2016·Journal of Molecular Endocrinology·Kent WehmeierMichael J Haas
Apr 25, 2012·Rheumatic Diseases Clinics of North America·J Wesley Pike, Mark B Meyer
Oct 22, 2009·Biogerontology·Daniel P Hayes

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