In vitro bioartificial skin culture model of tissue rejection and inflammatory/immune mechanisms

Transplantation Proceedings
L F StrandeC W Hewitt

Abstract

We hypothesized that an in vitro bioartificial skin rejection model using living LSEs grown in tissue culture could be developed for the study of autologous, allogenic, and/or xenogeneic inflammatory/immune mechanisms and topical immunosuppressive drugs. Human fibroblasts were mixed with type 1 rat-tail collagen to form a matrix (4 to 5 days), on which human keratinocytes were seeded. After a keratinocyte monolayer formed, CT cultures were raised to the air-liquid interface for continued growth. In the REJ LSE model, immunocytes isolated from human blood were seeded on top of the NHEK monolayer at the time of air-lifting. Thickness measurements of the acellular keratin and keratinocyte layers, and nuclear/cytoplasmic ratios, in both CT and REJ were made using digital image analysis. Immunostaining with anticytokeratin demonstrated a viable, keratin-producing epidermal layer; staining with anti-TGF-beta suggested a role for this cytokine in the rejection or wound-healing process. The LSE appeared histologically similar to normal human epidermis. Immunocytes added to the REJ cultures caused an obvious rejection response and were clearly identifiable in the gels as CD45+ staining cells. The LSE model appears promising for the stud...Continue Reading

References

Mar 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·E BellC Merrill
Jun 1, 1991·The British Journal of Dermatology·I A McKay, I M Leigh

❮ Previous
Next ❯

Citations

Mar 3, 2011·Archives of Dermatological Research·T Hodgkinson, A Bayat
Dec 28, 1999·Tissue Engineering·E J DoolinC Hewitt
Jul 12, 2005·The Journal of Burn Care & Rehabilitation·David G Greenhalgh
Oct 30, 2009·Journal of the Royal Society, Interface·Rostislav V ShevchenkoS Elizabeth James
Nov 22, 2011·Clinics in Plastic Surgery·Florian GroeberKatja Schenke-Layland
Jul 10, 2001·The Journal of Investigative Dermatology·A KernJ Mansbridge
Jan 19, 2011·Advanced Drug Delivery Reviews·Florian GroeberKatja Schenke-Layland

❮ Previous
Next ❯

Related Concepts

Related Feeds

Allogenic & Autologous Therapies

Allogenic therapies are generated in large batches from unrelated donor tissues such as bone marrow. In contrast, autologous therapies are manufactures as a single lot from the patient being treated. Here is the latest research on allogenic and autologous therapies.