In vitro bioassay with enhanced sensitivity for human granulocyte colony-stimulating factor

Journal of Pharmaceutical and Biomedical Analysis
U HammerlingL Sjödin

Abstract

A method for the determination of human granulocyte colony-stimulating factor (hG-CSF) activity, based on stimulation of cellular proliferation, was developed using a subclone of the murine myeloid leukemia cell line NFS-60, with an improved sensitivity for hG-CSF, as indicator. The optimal range for quantitative analysis of hG-CSF was about 4-60 pg ml-1. The stimulatory effect was measured by a colorimetric microassay: the optical density of formazan, which is produced by viable cells from 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), was obtained by reading plates in a multi-channel photometer. The assay was designed as a five-dose parallel line test, employing three or four doses for potency determinations, which fulfil pharmacopoeial requirements for assay validity. Inter-assay relative standard deviation (RSD) varied between 5.2 and 12.0%. Most assay experiments revealed potencies within limits of error of 90-110% and the mean index of precision value was 0.057. The recently developed yeast cell-derived International Standard (88/502) served as a reference for activity of rhG-CSF. Specificity of the assay was demonstrated by absence of response upon exposure to a panel of biomolecules, including recom...Continue Reading

References

Jun 1, 1977·Journal of Cellular Physiology·T M DexterL G Lajtha
Sep 8, 1975·Biochimica Et Biophysica Acta·R Cecil, G B Robinson
Jan 1, 1992·Clinical Immunology and Immunopathology·L A BoxerS Emerson
Jul 2, 1992·The New England Journal of Medicine·G J Lieschke, A W Burgess
Jul 9, 1992·The New England Journal of Medicine·G J Lieschke, A W Burgess
Aug 1, 1992·Journal of Pharmaceutical and Biomedical Analysis·U HammerlingL Sjödin
Mar 31, 1989·Journal of Immunological Methods·H MotojimaM Fukushima
Jun 1, 1989·The Journal of Pharmacy and Pharmacology·L SjödinE Viitanen
Apr 1, 1989·Trends in Pharmacological Sciences·G MorstynJ Cebon
Feb 24, 1989·Journal of Immunological Methods·W E PullmanA J Hapel
Oct 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·K L HolmesJ N Ihle
Jul 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·Y WeinsteinE P Reddy
Oct 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·C P ChiuF Lee
Oct 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·M TsuchiyaS Nagata
Nov 6, 1986·Journal of Immunological Methods·H TadaK Tsukamoto
Mar 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·K WelteM A Moore
Aug 1, 1966·Proceedings of the National Academy of Sciences of the United States of America·Y IchikawaL Sachs
Feb 10, 2006·Proceedings of the National Academy of Sciences of the United States of America·Yajing LiKevin Redding
Sep 10, 2011·Environmental Science & Technology·Lisette de HoopA Jan Hendriks

❮ Previous
Next ❯

Citations

Oct 1, 1998·Toxicology in Vitro : an International Journal Published in Association with BIBRA·U Hammerling, L Sjödin
Apr 2, 2005·Biotechnology Progress·Simona JevsevarViktor Menart
Dec 3, 2008·Microbial Cell Factories·Spela PeternelRadovan Komel
Jul 20, 2010·Biologicals : Journal of the International Association of Biological Standardization·A SkrlinS Marinc
May 19, 2010·Journal of Pharmaceutical and Biomedical Analysis·Ana SkrlinDomagoj Runac
Apr 25, 2019·Nature Biomedical Engineering·Rajani AdigaAdil Zuber

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.