PMID: 11912450Mar 26, 2002Paper

In vitro biocompatibility evaluation of naturally derived and synthetic biomaterials using normal human bladder smooth muscle cells

The Journal of Urology
J L ParienteAnthony Atala

Abstract

Tissue engineering of the urinary tract often requires the use of various biomaterials. Adequate biomaterial biocompatibility is necessary for successful urinary reconstruction. In this study using a primary normal human bladder smooth muscle cell culture system we evaluated the in vitro biocompatibility of a number of naturally derived biomaterials, including bladder submucosa, small intestinal submucosa, collagen and alginate, and polymeric biomaterials, including polyglycolic acid, poly(L-lactic acid) and poly(lactic-co-glycolic acid, which have been used for urinary reconstruction experimentally or clinically. To determine the cytotoxic and bioactive effects of bladder submucosa, small intestinal submucosa, collagen, alginate, polyglycolic acid, poly(L-lactic acid) and poly(lactic-co-glycolic acid) we measured cell viability, metabolic activity, apoptotic properties and DNA synthesis activity with 4 types of assays, namely Neutral Red (Sigma Chemical Co., St. Louis, Missouri), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (Sigma Chemical Co.), apoptotic activity and tritiated thymidine incorporation (Dupont NEN, Boston, Massachusetts) assays. Normal human bladder smooth muscle cells were cultured with the ex...Continue Reading

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Citations

Jul 3, 2008·World Journal of Urology·Byung-Soo KimJames J Yoo
Feb 3, 2011·Journal of Materials Science. Materials in Medicine·Daniel EberliJames J Yoo
Mar 22, 2003·Current Urology Reports·Adam R MetwalliBradley P Kropp
Jan 23, 2003·Current Urology Reports·Darren T BeikoJohn D Denstedt
Nov 13, 2012·The Journal of Surgical Research·Wenbiao LiaoYunhe Xiong
Dec 13, 2002·The Journal of Urology·Abdel W El-KassabyAnthony Atala
Jul 16, 2005·Expert Opinion on Biological Therapy·Anthony Atala
Feb 12, 2004·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Anthony Atala
Sep 21, 2007·International Journal of Urology : Official Journal of the Japanese Urological Association·Dah-Shyong YuSun-Yran Chang
May 5, 2009·International Braz J Urol : Official Journal of the Brazilian Society of Urology·Andre L A DomingosAntonio C P Martins
May 6, 2004·The Journal of Urology·Darren T BeikoJohn D Denstedt
Mar 3, 2004·Human & Experimental Toxicology·N A MotsoaneP J Becker
Apr 11, 2018·Journal of Biomedical Materials Research. Part a·Mehdi RazaviAvnesh S Thakor
Feb 14, 2006·Nature Clinical Practice. Urology·Anthony Atala
Mar 23, 2012·Journal of Tissue Engineering and Regenerative Medicine·Maya HorstDaniel Eberli
Feb 28, 2007·Journal of Biomaterials Science. Polymer Edition·Suk Ho BhangByung-Soo Kim
Nov 24, 2011·Chonnam Medical Journal·Jennifer L OlsonJames J Yoo
Feb 5, 2005·Journal of Biomedical Materials Research. Part a·Devanathan RaghavanSundararajan V Madihally
Nov 27, 2007·Journal of Tissue Engineering and Regenerative Medicine·Anthony Atala
Nov 19, 2005·Expert Review of Medical Devices·Anthony Atala, Chester Koh
Jun 23, 2007·Journal of Biomedical Materials Research. Part a·Sang Jin LeeJoel Stitzel
Jul 15, 2011·Journal of Tissue Engineering and Regenerative Medicine·Alexander Huber, Stephen F Badylak
May 7, 2008·Biomedical Materials·Michael McManusGary Bowlin

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