Abstract
Tissue engineering of the urinary tract often requires the use of various biomaterials. Adequate biomaterial biocompatibility is necessary for successful urinary reconstruction. In this study using a primary normal human bladder smooth muscle cell culture system we evaluated the in vitro biocompatibility of a number of naturally derived biomaterials, including bladder submucosa, small intestinal submucosa, collagen and alginate, and polymeric biomaterials, including polyglycolic acid, poly(L-lactic acid) and poly(lactic-co-glycolic acid, which have been used for urinary reconstruction experimentally or clinically. To determine the cytotoxic and bioactive effects of bladder submucosa, small intestinal submucosa, collagen, alginate, polyglycolic acid, poly(L-lactic acid) and poly(lactic-co-glycolic acid) we measured cell viability, metabolic activity, apoptotic properties and DNA synthesis activity with 4 types of assays, namely Neutral Red (Sigma Chemical Co., St. Louis, Missouri), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (Sigma Chemical Co.), apoptotic activity and tritiated thymidine incorporation (Dupont NEN, Boston, Massachusetts) assays. Normal human bladder smooth muscle cells were cultured with the ex...Continue Reading
References
Jan 1, 1975·Biomaterials, Medical Devices, and Artificial Organs·R G Mason, B A Shinoda
Mar 1, 1990·Biotechnology Progress·S S Ozturk, B O Palsson
Jan 1, 1987·Urological Research·M J MonsourR Scott
Mar 11, 1983·Journal of Immunological Methods·C R Parish, A Müllbacher
Dec 16, 1983·Journal of Immunological Methods·T Mosmann
Jul 1, 1980·In Vitro·E Tiffany-Castiglioni, J R Perez-Polo
Jan 1, 1996·The Journal of Urology·J D VaughtK B Thor
Apr 1, 1993·Biomaterials·G CiapettiA Pizzoferrato
Jun 1, 1996·The Journal of Urology·B P KroppK B Thor
Apr 1, 1996·Biomaterials·A A Ignatius, L E Claes
Mar 1, 1997·The Journal of Burn Care & Rehabilitation·V LattariR R Barrette
Apr 1, 1997·Biomaterials·L Shapiro, S Cohen
Mar 12, 1998·Urology·J J YooA Atala
Sep 8, 1998·Urology·J L ParienteM Le Guillou
Jan 23, 1999·Tissue Engineering·S F BadylakP W Snyder
Mar 3, 1999·Nature Biotechnology·F OberpenningA Atala
Mar 23, 1999·The Urologic Clinics of North America·R T Kershen, A Atala
Dec 9, 1993·Clinical Materials·A PizzoferratoL Savarino
Aug 24, 1999·The Journal of Urology·A AtalaA B Retik
Aug 24, 1999·The Journal of Urology·D A Diamond, A A Caldamone
Sep 2, 1999·Experimental Cell Research·B S KimD J Mooney
Sep 4, 1999·Urology·F ChenA Atala
Jan 20, 2000·Dimensions of Critical Care Nursing : DCCN·M E WinfreyM T Hegarty
Mar 18, 2000·Biomaterials·J L ParienteM Le Guillou
Mar 29, 2000·Journal of Endourology·A Atala
Apr 15, 2000·World Journal of Urology·B S KimA Atala
Jun 6, 2000·Advanced Drug Delivery Reviews·M S Shive, J M Anderson
Citations
Jul 3, 2008·World Journal of Urology·Byung-Soo KimJames J Yoo
Feb 3, 2011·Journal of Materials Science. Materials in Medicine·Daniel EberliJames J Yoo
Mar 22, 2003·Current Urology Reports·Adam R MetwalliBradley P Kropp
Jan 23, 2003·Current Urology Reports·Darren T BeikoJohn D Denstedt
Dec 11, 2003·Journal of Pediatric Surgery·Yuka NakanishiMichio Kaneko
Nov 19, 2003·Clinics in Plastic Surgery·Anthony Atala
Nov 13, 2012·The Journal of Surgical Research·Wenbiao LiaoYunhe Xiong
Nov 9, 2010·Biomaterials·Shaofeng WuYuanyuan Zhang
Mar 6, 2010·Biotechnology and Applied Biochemistry·Suk Ho BhangByung-Soo Kim
May 9, 2014·Organogenesis·Joao Paulo ZambonFernando G Almeida
Dec 13, 2002·The Journal of Urology·Abdel W El-KassabyAnthony Atala
Jul 16, 2005·Expert Opinion on Biological Therapy·Anthony Atala
Feb 19, 2010·Journal of Biomedical Materials Research. Part a·Chao FengJie Chen
Feb 12, 2004·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Anthony Atala
Sep 21, 2007·International Journal of Urology : Official Journal of the Japanese Urological Association·Dah-Shyong YuSun-Yran Chang
May 5, 2009·International Braz J Urol : Official Journal of the Brazilian Society of Urology·Andre L A DomingosAntonio C P Martins
May 6, 2004·The Journal of Urology·Darren T BeikoJohn D Denstedt
Mar 3, 2004·Human & Experimental Toxicology·N A MotsoaneP J Becker
Apr 11, 2018·Journal of Biomedical Materials Research. Part a·Mehdi RazaviAvnesh S Thakor
Feb 14, 2006·Nature Clinical Practice. Urology·Anthony Atala
Mar 23, 2012·Journal of Tissue Engineering and Regenerative Medicine·Maya HorstDaniel Eberli
Feb 28, 2007·Journal of Biomaterials Science. Polymer Edition·Suk Ho BhangByung-Soo Kim
Nov 24, 2011·Chonnam Medical Journal·Jennifer L OlsonJames J Yoo
Jul 13, 2004·The Journal of Urology·D N WoodC H Fry
Jan 11, 2019·Nanomaterials·Priscila SiqueiraVagner Roberto Botaro
Feb 5, 2005·Journal of Biomedical Materials Research. Part a·Devanathan RaghavanSundararajan V Madihally
Nov 27, 2007·Journal of Tissue Engineering and Regenerative Medicine·Anthony Atala
Nov 19, 2005·Expert Review of Medical Devices·Anthony Atala, Chester Koh
Jun 23, 2007·Journal of Biomedical Materials Research. Part a·Sang Jin LeeJoel Stitzel
Jul 15, 2011·Journal of Tissue Engineering and Regenerative Medicine·Alexander Huber, Stephen F Badylak
Jan 4, 2012·Macromolecular Bioscience·Suk Ho BhangHeungsoo Shin
Mar 29, 2014·Journal of Tissue Engineering and Regenerative Medicine·Fanrong PuJohn A Hunt
May 7, 2008·Biomedical Materials·Michael McManusGary Bowlin