In vitro bioconversion of chitin to pyruvate with thermophilic enzymes

Journal of Bioscience and Bioengineering
Kohsuke HondaHisao Ohtake

Abstract

Chitin is the second most abundant organic compound on the planet and thus has been regarded as an alternative resource to petroleum feedstocks. One of the key challenges in the biological conversion of biomass-derived polysaccharides, such as cellulose and chitin, is to close the gap between optimum temperatures for enzymatic saccharification and microbial fermentation and to implement them in a single bioreactor. To address this issue, in the present study, we aimed to perform an in vitro, one-pot bioconversion of chitin to pyruvate, which is a precursor of a wide range of useful metabolites. Twelve thermophilic enzymes, including that for NAD+ regeneration, were heterologously produced in Escherichia coli and semi-purified by heat treatment of the crude extract of recombinant cells. When the experimentally decided concentrations of enzymes were incubated with 0.5 mg mL-1 colloidal chitin (equivalent to 2.5 mM N-acetylglucosamine unit) and an adequate set of cofactors at 70°C, 0.62 mM pyruvate was produced in 5 h. Despite the use of a cofactor-balanced pathway, determination of the pool sizes of cofactors showed a rapid decrease in ATP concentration, most probably due to the thermally stable ATP-degrading enzyme(s) derived fr...Continue Reading

References

Dec 5, 2000·Nature Structural Biology·S YokoyamaS Kuramitsu
Dec 18, 2002·Proceedings of the National Academy of Sciences of the United States of America·Haiyu ZhangArthur Kornberg
Apr 23, 2003·Critical Reviews in Food Science and Nutrition·Józef Synowiecki, Nadia Ali Al-Khateeb
Sep 13, 2005·Current Opinion in Biotechnology·Lee R LyndMark Laser
Oct 4, 2005·Journal of Bacteriology·Takeshi TanakaTadayuki Imanaka
Mar 5, 2008·BMB Reports·Soonbok KwonDongbin Lim
Sep 10, 2008·Proceedings of the National Academy of Sciences of the United States of America·A Joe ShawLee R Lynd
Dec 20, 2011·Current Opinion in Biotechnology·Daniel G OlsonLee R Lynd
Jun 4, 2014·Proceedings of the National Academy of Sciences of the United States of America·Daehwan ChungJanet Westpheling
Oct 31, 2014·Proceedings of the National Academy of Sciences of the United States of America·Igor W BogoradJames C Liao
Apr 8, 2015·Proceedings of the National Academy of Sciences of the United States of America·Joseph A RollinY-H Percival Zhang
Feb 26, 2016·Metabolic Engineering·Kohsuke HondaHisao Ohtake

❮ Previous
Next ❯

Citations

Jul 14, 2020·Critical Reviews in Biotechnology·Dominik Kopp, Anwar Sunna
Aug 10, 2019·Applied Microbiology and Biotechnology·Yohei HanataniKohsuke Honda
Dec 19, 2019·Frontiers in Bioengineering and Biotechnology·Dominik KoppAnwar Sunna
Oct 6, 2020·Critical Reviews in Biotechnology·Hongge Chen, Yi-Heng P Job Zhang
Jan 9, 2018·Current Opinion in Biotechnology·Kristen M WildingBradley C Bundy
Oct 25, 2017·Synthetic and Systems Biotechnology·Hironori TaniguchiKohsuke Honda
May 16, 2021·Applied and Environmental Microbiology·Gladwin Suryatin AlimKohsuke Honda

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Applied Biochemistry and Biotechnology
Ali AkbarzadehSakineh Moshtaghi
Seikagaku. The Journal of Japanese Biochemical Society
Satoshi Akanuma, Akihiko Yamagishi
© 2022 Meta ULC. All rights reserved