In vitro biofilm formation on resin-based composites cured under different surface conditions

Journal of Dentistry
Andrei IonescuEugenio Brambilla

Abstract

The interfacial conditions occurring during light-curing procedures of resin-based composites (RBCs) influence their surface properties and therefore the biological behavior of the material. This study aimed to evaluate the influence of different surface curing conditions on in vitro biofilm formation by Streptococcus mutans and mixed oral microflora, in the presence or absence of surface salivary pre-conditioning. Two nanohybrid RBCs and four interfacial curing conditions (open air, argon, nitrogen and glycerin) were evaluated. Surface roughness (SR), surface elemental composition (energy-dispersive X-ray spectrometry, EDS) and biofilm formation (S. mutans and oral microcosm) were assessed. Surfaces were observed using scanning electron microscopy (SEM). Microbiological tests were performed with and without saliva pre-conditioning of the surfaces. EDS analysis was performed before and after biofilm formation, and biofilm morphology was evaluated using confocal laser scanning microscopy (CLSM). Data were analyzed using multi-way ANOVA and Tukey post-hoc test (p < 0.05). Interfacial curing conditions significantly influenced SR depending on the tested RBC. EDS analysis showed that surface elemental composition was significantly ...Continue Reading

Citations

Oct 29, 2020·Clinical Oral Investigations·Torsten SterzenbachMatthias Hannig
Jun 3, 2021·International Journal of Environmental Research and Public Health·Adriano CasagliaLoredana Cerroni
Aug 7, 2021·Dentistry Journal·Gaetano PaoloneGiuseppe Cantatore

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