PMID: 11312798Apr 21, 2001Paper

In vitro biogeneration of pure thiosulfinates and propanethial-S-oxide

Journal of Agricultural and Food Chemistry
C Shen, K L Parkin

Abstract

A model reaction system was developed for generating pure thiosulfinates and propanethial-S-oxide (PTSO) using an isolated alliinase (EC 4.4.1.4) and isolated or synthetic alk(en)yl-L-cysteine sulfoxides (ACSO). Reaction yields ranged from 30 to 60% after 3 h at 21-23 degrees C, and organosulfur reaction products were extracted into CHCl3 to yield product preparations of controlled composition. A pure thiosulfinate or PTSO was derived from a single ACSO, and a preparation containing a mixture of four thiosulfinate species was derived from reaction mixtures employing binary ACSO substrate systems. Identities of homologous thiosulfinates and PTSO were confirmed by 1H NMR. This approach has the potential to be used as a preparative tool for yielding pure thiosulfinates and PTSO to facilitate the study of chemical and biological properties of this group of compounds or as a means to study the dynamics of organosulfur chemistry in preparations from Allium spp.

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Citations

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Sep 14, 2012·Bioscience, Biotechnology, and Biochemistry·Noriya MasamuraShinsuke Imai
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Jul 9, 2016·Preventive Nutrition and Food Science·Hyun Jung LeeHyeon-Son Choi
Sep 16, 2011·Journal of the Science of Food and Agriculture·Jungeun ChoSeung Koo Lee
Feb 2, 2006·Journal of Agricultural and Food Chemistry·Shinsuke ImaiHiroshi Sawada
Jan 22, 2010·Journal of Agricultural and Food Chemistry·Guodong ZhangKirk L Parkin
Apr 18, 2002·Journal of Agricultural and Food Chemistry·Hang Xiao, Kirk L Parkin
Apr 18, 2002·Journal of Agricultural and Food Chemistry·Cunxi ShenKirk L Parkin
Apr 18, 2002·Journal of Agricultural and Food Chemistry·Cunxi ShenKirk L Parkin
Mar 16, 2012·Journal of Agricultural and Food Chemistry·Victor Manuel BeatoAlfredo Montaño
Jan 17, 2008·Journal of Agricultural and Food Chemistry·Dan WangGuanghua Zhao
Mar 2, 2006·Journal of Agricultural and Food Chemistry·Makoto IchikawaKazuhisa Ono
Jun 22, 2006·Journal of Agricultural and Food Chemistry·Makoto IchikawaKazuhisa Ono

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