PMID: 8583012Jul 1, 1995Paper

In-vitro biopotency and glycoform distribution of recombinant human follicle stimulating hormone (Org 32489), Metrodin and Metrodin-HP

Human Reproduction
A LambertW R Robertson

Abstract

In this study the in-vitro biopotency and glycoform distribution of human recombinant follicle stimulating hormone (FSH, Org 32489) has been assessed. The biopotency of recombinant FSH was studied using animal (rat Sertoli) and human (granulosa-lutein) cell models. Recombinant FSH, as measured in the rat Sertoli cell assay, was more potent than the urinary preparations Metrodin, Metrodin-HP and IS 70/45 with half maximal stimulation (ED50; mean +/- SEM, n > 3) occurring at 2.2 +/- 0.5 IU/I (recombinant FSH), 4.7 +/- 1.1 IU/I (Metrodin), 13.2 +/- 0.7 IU/I (Metrodin-HP) and 6.4 +/- 0.3 IU/I (IS 70/45); the pituitary preparation IRP 83/575 had an ED50 of 10.4 +/- 0.1 IU/I. Using human granulosa-lutein cells, cultured for up to 4 days in the absence of exogenous steroid precursors, recombinant FSH was either without effect (three out of five patients) or inhibited both oestradiol and progesterone secretion. FSH (83/575) was without effect on oestradiol with preparations from any of the patients but slightly stimulated (134 +/- 8%; mean +/- SEM, P < 0.05) progesterone production at the highest dose (80 IU/I). The distribution of FSH isoforms, assessed by polyclonal radioimmunoassay, following chromatofocusing over the ranges pH < 3....Continue Reading

Citations

Apr 18, 2001·Molecular and Cellular Endocrinology·S CreusS Campo
Mar 20, 2002·Molecular and Cellular Endocrinology·J Barrios-De-TomasiA Ulloa-Aguirre
Jul 19, 2008·Anesthesia and Analgesia·Kamila VagnerovaPatricia D Hurn
Feb 18, 2010·Biologics : Targets & Therapy·Ahmed Gibreel, Siladitya Bhattacharya
Aug 31, 2004·Reproductive Biomedicine Online·Claus Yding AndersenMadelon van Wely
Apr 7, 2009·Gynécologie, obstétrique & fertilité·J-C Emperaire
Jul 10, 2018·Endocrine Reviews·Ross C AndersonRobert P Millar
Jul 30, 1999·British Journal of Obstetrics and Gynaecology·C J HaydenA J Rutherford
Sep 10, 2015·The Cochrane Database of Systematic Reviews·Nienke S WeissMadelon van Wely
Mar 4, 2000·Endocrine Reviews·M P RoseA H Balen
Jan 17, 2019·The Cochrane Database of Systematic Reviews·Nienke S WeissMadelon van Wely
Mar 1, 2014·Gynecological Endocrinology : the Official Journal of the International Society of Gynecological Endocrinology·Joan-Carles ArceAntonio La Marca
May 20, 2005·Reproductive Biomedicine Online·Claudio WolfensonSonja Jespersen

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