In vitro cartilage formation by human adult stem cells from bone marrow stroma defines the sequence of cellular and molecular events during chondrogenesis

Proceedings of the National Academy of Sciences of the United States of America
Ichiro SekiyaDarwin J Prockop

Abstract

One approach to resolving the complexities of chondrogenesis is to examine simplified systems in vitro. We analyzed cartilage differentiation by human adult stem cells from bone marrow stroma. Marrow stromal cells were cultured as micromass pellets for 21 days in serum-free medium containing transforming growth factor (TGF)-beta3, dexamethasone, and bone morphogenetic protein (BMP)-6. Assays for pulse-labeled [3H]DNA and for total DNA indicated that there was little proliferation and a progressive loss of cells in the pellets. There were continuous increases in mRNAs for cartilage matrix (proteoglycans and COL2, -9, -10, and -11), receptors [fibroblast growth factor 2 (FGFR2) and parathyroid hormone-related peptide receptor (PTHrP-R)], and transcription factors (SOX5, -6, and -9) as demonstrated by histochemical and microarray assays. Reverse transcription-PCR assays for 11 mRNAs confirmed the microarray data. SOX4, vascular endothelial growth factor (VEGF), and matrix metalloproteinase 14 (MMP14) increased at day 1 and decreased thereafter, suggesting roles early in chondrogenesis. Also, forkhead, CD10, and MMP13 increased up to day 7 and decreased thereafter, suggesting roles in an intermediate stage of chondrogenesis. In add...Continue Reading

References

Oct 1, 1977·Developmental Biology·P B AhrensR S Reiter
Feb 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·S KinoshitaT Kishimoto
Oct 1, 1988·The Journal of Experimental Medicine·M LetarteR R McInnes
Mar 5, 1996·Proceedings of the National Academy of Sciences of the United States of America·J LeeW I Wood
Aug 1, 1997·Current Opinion in Genetics & Development·L Niswander
Jan 13, 1998·Current Opinion in Cell Biology·K D Irvine, T F Vogt
Feb 11, 1998·Experimental Cell Research·B JohnstoneJ U Yoo
May 6, 1998·Matrix Biology : Journal of the International Society for Matrix Biology·V Lefebvre, B de Crombrugghe
Dec 1, 1996·Nature Biotechnology·D J LockhartE L Brown
Jan 23, 1999·Nature Genetics·R J LipshutzD J Lockhart
Apr 2, 1999·Science·M F PittengerD R Marshak
Jul 15, 1999·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·T SakouP Ten Dijke
Dec 29, 2000·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·S ReppeK M Gautvik
Mar 10, 2001·Journal of Cellular Biochemistry·K HanadaB Johnstone
May 26, 2001·The Journal of Endocrinology·I SekiyaM Noda
Jun 8, 2001·Biochemical and Biophysical Research Communications·I SekiyaD J Prockop
Jul 17, 2001·Journal of Cellular Biochemistry. Supplement·I SekiyaM Noda
Oct 5, 2001·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·A NifujiM Noda

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Citations

May 8, 2007·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Hou-De ZhouEr-Yuan Liao
Apr 1, 2009·Knee Surgery, Sports Traumatology, Arthroscopy : Official Journal of the ESSKA·Hideyuki KogaIchiro Sekiya
Dec 19, 2009·Knee Surgery, Sports Traumatology, Arthroscopy : Official Journal of the ESSKA·Rune B JakobsenJan E Brinchmann
Aug 23, 2012·Histochemistry and Cell Biology·Siyuan LiClare E Hughes
Jul 11, 2013·Histochemistry and Cell Biology·Mohamadreza Baghaban EslaminejadMaryam Shahhoseini
Aug 11, 2005·Cell and Tissue Research·Shizuko IchinoseIchiro Sekiya
Nov 10, 2007·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·Fangming Lin
Dec 2, 2009·Apoptosis : an International Journal on Programmed Cell Death·Chien-Yuan WangShih-Chieh Hung
Aug 2, 2007·Journal of Molecular Histology·Yash M KolambkarRobert E Guldberg
Aug 13, 2011·Journal of Materials Science. Materials in Medicine·Mohammad Kazem GhahramanpoorMohamadreza Baghaban Eslaminejad
Apr 4, 2007·Pharmaceutical Research·Mohamed HaiderKam W Leong
May 16, 2008·Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban·Fenghua TaoFeng Pan
Jun 11, 2009·Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban·Weihua HuAnmin Chen
Nov 5, 2003·Gene·Kathryn L MaschhoffH Scott Baldwin
Nov 19, 2003·Clinics in Plastic Surgery·Marc H Hedrick, Eric J Daniels
Jun 18, 2003·Current Opinion in Pharmacology·Hairong Peng, Johnny Huard
May 24, 2008·Cell Death and Differentiation·L Ricci-VitianiR De Maria
May 24, 2007·Molecular Therapy : the Journal of the American Society of Gene Therapy·Terri ZachosAlicia Bertone
Feb 13, 2010·Proceedings of the National Academy of Sciences of the United States of America·Ulf KrauseCarl A Gregory
Sep 2, 2010·Human Gene Therapy·Andrea Augello, Cosimo De Bari
Nov 16, 2006·Stem Cells and Development·Neng-Sheng YeYi-Ming Wang
May 6, 2008·Stem Cells and Development·Gwendolyn M HobenKyriacos A Athanasiou
Dec 24, 2008·Stem Cells and Development·Tong Ming LiuBing Lim
Jan 11, 2012·Stem Cells and Development·Verena DexheimerWiltrud Richter
Jan 11, 2013·Stem Cells and Development·Serena GuidottiRosa Maria Borzì

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