PMID: 9641630Jun 26, 1998Paper

In vitro cellular responses to bioerodible particles loaded with recombinant human bone morphogenetic protein-2

Journal of Biomedical Materials Research
D A PuleoP P DeLuca

Abstract

Porous 50:50 poly(d,l lactide-co-glycolide) microspheres containing varying amounts of "free" recombinant human bone morphogenetic protein-2 (rhBMP-2) were evaluated for their ability to induce/enhance expression of osteoblastic characteristics by pluripotent mesenchymal cells in vitro. "Free" protein (Fp) is defined as protein present on the surface and within the porous matrix of the microspheres. Four preparations of bioerodible particles (BEP) were used: blank--without rhBMP-2; low Fp--24 microg of free rhBMP-2 per g of particles; medium Fp--403 microg/g; and high Fp--884 microg/g. C3H10T1/2 cells (C3H) and bone marrow stromal cells (BMC) were cultured with 1 mg of BEP for up to 4 weeks, and cell growth and expression of osteogenic responses were determined weekly. For both cell types, control cultures (neither BEP nor rhBMP-2) and cultures with blank BEP exhibited no or minimal osteoblastic characteristics. Compared to control and blank BEP cultures, C3H cells responded to particles having medium and high amounts of free rhBMP-2 with increased cell growth and alkaline phosphatase activity, but osteocalcin secretion and mineralization were not markedly influenced. Low Fp BEP enhanced only the alkaline phosphatase activity o...Continue Reading

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Citations

Mar 29, 2000·Journal of Biomedical Materials Research·L LuA G Mikos
Nov 18, 2005·European Spine Journal : Official Publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·P I J M Wuisman, T H Smit
Aug 25, 2001·Biomaterials·V I SikavitsasA G Mikos
Aug 8, 2001·Journal of Controlled Release : Official Journal of the Controlled Release Society·B H WooP P DeLuca
Sep 1, 2000·Advanced Drug Delivery Reviews·C A Kirker-Head
Aug 3, 2000·Journal of Biomechanical Engineering·J B OldhamM J Yaszemski
Jul 23, 2004·Expert Opinion on Biological Therapy·J Kent Leach, David J Mooney
Jun 6, 2013·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Mohammad Reza ZarrinkalamRobert J Moore
Feb 27, 2010·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·In-Ho BaeJeong-Tae Koh
Jun 24, 2005·Journal of Controlled Release : Official Journal of the Controlled Release Society·P Q RuhéJ A Jansen
Nov 17, 2004·Journal of Controlled Release : Official Journal of the Controlled Release Society·Elizabeth L HedbergAntonios G Mikos
Aug 1, 2008·Journal of Biomaterials Applications·Shanshan ShiYufeng Zhang
Apr 2, 2004·Journal of Biomedical Materials Research. Part a·A T Raiche, D A Puleo
Jul 20, 2007·Journal of Biomedical Materials Research. Part a·Maria KarpovAnna M Osyczka
Jan 25, 2019·The Journal of Craniofacial Surgery·Filiberto MastrangeloEitan Mijiritsky
Aug 20, 2003·The Journal of Bone and Joint Surgery. American Volume·P Quinten RuheAntonios G Mikos
Jul 11, 2020·Advanced Biosystems·Sara E StreckerNatalie Artzi

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