In vitro cellular responses to cytokines and erythropoietin

Toxicology in Vitro : an International Journal Published in Association with BIBRA
U Hammerling, L Sjödin

Abstract

Cytokines and polypeptide hormones act through high-affinity binding to cognate transmembrane receptor molecules, expressed on target cells. The impact of such ligand molecules is conveyed to the cell nucleus by specific signal transduction mechanisms and is ultimately manifested as changes in gene expression, largely accomplished by transcription-regulatory factors. Depending on target cell maturation and receptor signalling pathways, cell-cycle progression or growth inhibition may follow from ligand/receptor interactions. We have employed cellular growth as an endpoint for potency determination of several human bioactive substances, such as interferons (IFNs), IL-2, G-CSF, GM-CSF and erythropoietin (Epo), using murine or human cell lines as indicators. The conversion of the tetrazolium salt MTT by mitochondrial reductase to blue formazan served as an endpoint in such estimations. In addition to a cellular growth suppression IFN assay, a reporter gene-modified human glioblastoma line was devised to provide an implement for high-throughput potency assessment of interferons. The bioassay systems were all designed according to the parallel line assay model and were subjected to extensive validation procedures. Both intra- and int...Continue Reading

References

Jul 14, 1977·Nature·S Gillis, K A Smith
Aug 1, 1992·Journal of Pharmaceutical and Biomedical Analysis·U HammerlingL Sjödin
Oct 17, 1991·The New England Journal of Medicine·J A Romijn
Jan 1, 1990·Biologicals : Journal of the International Association of Biological Standardization·T NedermanL Sjödin
Jul 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·Y WeinsteinE P Reddy
Jan 1, 1995·Journal of Pharmaceutical and Biomedical Analysis·U HammerlingL Sjödin
Jan 1, 1993·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·N Kobayashi
Aug 26, 1998·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·U HammerlingL Sjödin

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Citations

May 16, 2000·Tropical Medicine & International Health : TM & IH·G BiembaG Weiss
Jun 4, 2016·Alternatives to Laboratory Animals : ATLA·Francine T MachadoSérgio L Dalmora

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