PMID: 11315510Apr 24, 2001Paper

In vitro characterisation of a monovalent and bivalent form of a fully human anti Ep-CAM phage antibody

Cancer Immunology, Immunotherapy : CII
R C RooversH R Hoogenboom

Abstract

Antibodies to tumour-associated antigens are increasingly being used as targeting vehicles for the visualisation and for therapy of human solid tumours. The epithelial cell adhesion molecule (Ep-CAM) is an antigen that is overexpressed on a variety of human solid tumours and constitutes an attractive target for immunotargeting. We set out to obtain fully human antibodies to this antigen by selecting from a large antibody repertoire displayed on bacteriophages. Two single-chain variable antibody fragments (scFv) were identified that specifically bound recombinant antigen in vitro. One of the selected antibodies (VEL-1) cross-reacted with extracellular matrix components in immunohistochemistry of colon carcinoma, whereas the other scFv (VEL-2) specifically recognised colon cancer cells. The latter antibody was further characterised with respect to epitope specificity and kinetics of antigen-binding. It showed no competition with the well-characterised anti Ep-CAM MOC-31 monoclonal antibody and had an off-rate of 5 x 10(-2) s-1. To obtain an antibody format more suitable for in vivo tumour targeting and to increase the apparent affinity through avidity, the genes of scFv VEL-2 were re-formatted by fusion to a human (gamma 1) hinge...Continue Reading

Citations

Aug 31, 2004·Journal of Clinical Pathology·K A SmithJ Greenman
Oct 16, 2001·Drugs & Aging·S Matzku, M Zöller
Jun 2, 2006·Cancer Immunology, Immunotherapy : CII·Rob C RooversPaul M P van Bergen en Henegouwen
Dec 1, 2001·Expert Opinion on Biological Therapy·P J Hudson, C Souriau
Nov 26, 2003·Histopathology·T García-CaballeroA Beiras
Aug 8, 2006·Biochemical and Biophysical Research Communications·Christine MazuetNathalie Blin
Dec 26, 2002·Journal of Molecular Recognition : JMR·Rebecca L Rich, David G Myszka
Apr 4, 2003·International Journal of Cancer. Journal International Du Cancer·Pauline WimbergerRainer Kimmig
Apr 2, 2002·World Journal of Gastroenterology : WJG·Feng-Tian HeYan Zhang
Aug 14, 2002·World Journal of Gastroenterology : WJG·Yong-Zhan NieDai-Ming Fan

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacteriophage: Phage Therapy

Phage therapy uses bacterial viruses (bacteriophages) to treat bacterial infections and is widely being recognized as an alternative to antibiotics. Here is the latest research.

Antibody Repertoire Diversity

Antibody repertoire diversity and its role during natural infection is a prerequisite for molecular and structural elucidation of functionally protective immunity. Discover the latest insights into antibody diversity here.

© 2021 Meta ULC. All rights reserved