In vitro characteristics and in vivo immunosuppressive activity of compact bone-derived murine mesenchymal progenitor cells

Stem Cells
Zikuan GuoNing Mao

Abstract

In contrast to the considerable amount of data that documents the biological properties of mesenchymal progenitor cells from human and other species, there is still paucity of information about mouse counterparts, as their purification and culture expansion procedures remain rudimentary. In the present study, murine mesenchymal progenitor cell (muMPC) culture was developed by explant culture of collagenase-digested bone fragments after removal of the released cells. During cultivation, fibroblastoid cells sprouted and migrated from the fragments, followed by adherent monolayer development. The cells exhibited homogenous surface antigen profile and presented in vitro multipotential differentiation along osteocyte, chondrocyte, and adipocyte lineages, as evaluated by matched cell or matrix staining and reverse transcription polymerase chain reaction techniques. Also, the surface antigenic epitope changed and potential of proliferation and multidifferentiation decreased with successive subculturing. Functional investigations demonstrated that these cells supported in vitro hematopoiesis and suppressed lymphocyte cell proliferation triggered by ConA or allogeneic splenocytes. Furthermore, muMPCs prolonged the mean survival time of ...Continue Reading

References

Feb 11, 1998·Experimental Cell Research·B JohnstoneJ U Yoo
Apr 3, 1998·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·M E NuttallM Gowen
Apr 2, 1999·Science·M F PittengerD R Marshak
Oct 20, 2000·Clinical Orthopaedics and Related Research·J D MoscaB M Sandmaier
Feb 2, 2002·Experimental Hematology·Amelia BartholomewRonald Hoffman
Apr 16, 2002·Cytotherapy·B ShortP J Simmons
Jun 22, 2002·Nature·Yuehua JiangCatherine M Verfaillie
Aug 6, 2002·Experimental Hematology·Douglas R MartinHenry J Baker
Oct 18, 2002·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Ulrich NöthRocky S Tuan
Oct 24, 2002·Blood·Caryn Y ItoWilliam L Stanford
May 7, 2003·Proceedings of the National Academy of Sciences of the United States of America·Mortaza BonyadiWilliam L Stanford
May 30, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Sarah E DuffShant Kumar
Jun 12, 2003·Experimental Cell Research·Giordano BianchiRodolfo Quarto
Oct 24, 2003·Nature·L M CalviD T Scadden
Nov 18, 2003·British Journal of Haematology·Lindolfo da Silva Meirelles, Nance Beyer Nardi
Apr 20, 2004·Experimental Cell Research·Philippe TropelFrançois Berger
Oct 20, 2004·Journal of Cellular and Molecular Medicine·D BakshR S Tuan
Oct 21, 2004·Blood·Sudeepta Aggarwal, Mark F Pittenger

❮ Previous
Next ❯

Citations

Jan 16, 2009·Bulletin of Experimental Biology and Medicine·A F TsybE V Agaeva
Feb 4, 2011·Human Gene Therapy·Jakub TolarArmand Keating
Aug 8, 2013·World Journal of Gastroenterology : WJG·Qian-Qian ChenJun Wan
Oct 25, 2013·British Medical Bulletin·Suzanne M WattJaap Jan Zwaginga
May 16, 2012·Biochemical and Biophysical Research Communications·Dahu LiLingqiang Zhang
Mar 1, 2012·Differentiation; Research in Biological Diversity·Maria Kuzma-KuzniarskaPatricia Murray
Apr 8, 2010·Cytotherapy·Yousof GheisariMasoud Soleimani
Oct 22, 2008·Transplantation Proceedings·J H SungS-J Kim
May 11, 2015·The Journal of Surgical Research·John P DavisGilbert R Upchurch
Oct 29, 2008·Cell Biochemistry and Function·Kun GaoYanrong Lu
Apr 25, 2007·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Marcus JägerRüdiger Krauspe
Mar 24, 2007·Stem Cells·Christina Holmes, William L Stanford
Nov 10, 2010·Cell Biochemistry and Function·Yanrong LuJingqiu Cheng
Dec 25, 2010·Birth Defects Research. Part C, Embryo Today : Reviews·Cosimo De BariAndrea Augello
Nov 23, 2010·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Xin-Long YanNing Mao
Sep 9, 2008·Biochemical and Biophysical Research Communications·Ji-De JinZi-Kuan Guo
Mar 27, 2007·Biology of Blood and Marrow Transplantation : Journal of the American Society for Blood and Marrow Transplantation·Andrea T BadilloAlan W Flake
Sep 3, 2016·Scientific Reports·Hsu-Tung LeeWoei-Cherng Shyu
Jun 12, 2012·Journal of Burn Care & Research : Official Publication of the American Burn Association·Emily R NelsonBenjamin Levi
Aug 16, 2014·World Journal of Stem Cells·Fei Li, Shao-Zhen Zhao
Aug 21, 2013·Circulation Research·Sang-Ging Ong, Joseph C Wu
Dec 30, 2006·Stroke; a Journal of Cerebral Circulation·Woei-Cherng ShyuHung Li

❮ Previous
Next ❯

Related Concepts

Related Feeds

Allogenic & Autologous Therapies

Allogenic therapies are generated in large batches from unrelated donor tissues such as bone marrow. In contrast, autologous therapies are manufactures as a single lot from the patient being treated. Here is the latest research on allogenic and autologous therapies.