In vitro characterization of chondrogenic cells isolated from chick embryonic muscle using peanut agglutinin affinity chromatography

Experimental Cell Research
E StringaR S Tuan

Abstract

Specific binding to the lectin, peanut agglutinin (PNA), has been reported in embryonic precartilage tissues, including the condensing limb bud blastema and the caudal half of the developing somite. The present study aimed to test the hypothesis that PNA-binding may be a surface characteristic of chondroprogenitor cells residing within noncartilage tissues, such as muscle, which have the potential of being induced to form cartilage, e.g., in the presence of bone matrix-derived factors. Day-14 chick embryonic pectoral muscle, which contained histochemically detectable PNA-binding cells, was dissociated into single cells (TM cells) and fractionated by PNA affinity chromatography into PNA-binding (PNA+) and nonbinding (PNA-) cells by PNA-Sepharose 6 MB affinity chromatography. The differentiation potential of the PNA-affinity fractionated cells in vitro was analyzed as a function of culture plating cell density. Immunohistochemistry of a number of cell-type-specific differentiation markers, including sarcomeric actin, collagen type II, and aggrecan core protein, demonstrated that PNA+ cells, when cultured as a micromass at high density (20 x 10(6) cells/ml), exhibited a chondrocyte-like phenotype, whereas the PNA-cells remained my...Continue Reading

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Citations

Feb 18, 2010·BMC Developmental Biology·Jamie L WoodSusan C Chapman
Nov 21, 1998·Microscopy Research and Technique·N J HickokR S Tuan
Feb 12, 2002·Developmental Dynamics : an Official Publication of the American Association of Anatomists·A Cevik TufanRocky S Tuan
Apr 7, 2006·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Lara LongobardiAnna Spagnoli
Sep 7, 2006·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Maria A Mello, Rocky S Tuan
Jun 2, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·A C Tufan, R S Tuan
Oct 14, 2004·Clinical Orthopaedics and Related Research·Rocky S Tuan
Sep 1, 2000·Osteoarthritis and Cartilage·A M DeLiseR S Tuan
Aug 14, 2003·Mechanisms of Development·Cynthia M Coleman, Rocky S Tuan

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