Abstract
Interleukin 26 (IL-26) gene has been identified in human, amphibian and teleost but not in rodents. It is well accepted that IL-26 was a crucial member of IL-10 family which acts as a pro-inflammatory cytokine in human. However, the role of IL-26 in regulating inflammation in lower vertebrates including teleost has not been defined yet. In the present study, grass carp IL-26 (gcIL-26) coding sequence was isolated and identified. Its chromosomal synteny was also analyzed, showing that gcIL-26 gene is flanked by IL-22 and IFN-γ genes with the same transcriptional orientation as seen in human, amphibian and zebrafish. Given that zebrafish and grass carp IL-26 shared relatively low amino acid identities with human IL-26, the functional roles of fish IL-26 are indispensable to be elucidated. Accordingly, recombinant gcIL-26 (rgcIL-26) was prepared by using Pichia pastoris expression system, and it was found to be partially glycosylated. Using grass carp head kidney leucocytes as cell model, rgcIL-26 displayed the bioactivity to stimulate the mRNA expression of some pro-inflammatory cytokines including IL-8, IL-1β and IL-6, while inhibit mRNA expression of an anti-inflammatory cytokine, IL-10. Moreover, rgcIL-26 also up-regulated ino...Continue Reading
References
Oct 1, 1982·Analytical Biochemistry·L C GreenS R Tannenbaum
Mar 23, 2000·Journal of Virology·A KnappeH Fickenscher
Mar 23, 2004·Annual Review of Immunology·Sidney PestkaPaul B Fisher
May 4, 2004·International Immunopharmacology·Helmut Fickenscher, Heide Pirzer
Jun 5, 2004·The Journal of Biological Chemistry·Simon HörHelmut Fickenscher
Jul 12, 2005·Molecular Immunology·Daisuke IgawaRam Savan
Aug 7, 2007·Nature Immunology·Nicholas J WilsonRene de Waal Malefyt
May 17, 2008·Gut·J DambacherS Brand
Aug 30, 2008·Immunogenetics·Z T Qi, P Nie
Feb 28, 2009·Clinical Chemistry·Stephen A BustinCarl T Wittwer
Apr 1, 2009·Fish & Shellfish Immunology·J M CovelloB F Nowak
May 18, 2010·Fish & Shellfish Immunology·Jung-Chen HsiehJyh-Yih Chen
Aug 24, 2010·Fish & Shellfish Immunology·Xinyan WangHong Zhou
Oct 16, 2010·Cytokine & Growth Factor Reviews·Raymond P DonnellyMark R Walter
Nov 30, 2010·Cytokine & Growth Factor Reviews·Robert Sabat
May 25, 2011·Developmental and Comparative Immunology·C J SecombesS Bird
Oct 12, 2012·PLoS Biology·Murielle CorvaisierPascale Jeannin
Jul 23, 2013·PloS One·Oliver BraumHelmut Fickenscher
Sep 24, 2013·Fish & Shellfish Immunology·Kun YangHong Zhou
Aug 6, 2014·Developmental and Comparative Immunology·Xiao YangHong Zhou
Oct 8, 2014·American Journal of Respiratory and Critical Care Medicine·Karlhans F CheAnders Lindén
Mar 26, 2015·Fish & Shellfish Immunology·He WeiHong Zhou
May 6, 2015·Nature Genetics·Yaping WangZuoyan Zhu
Jul 15, 2015·Nature Immunology·Stephan MellerMichel Gilliet
Jul 24, 2015·Journal of Innate Immunity·Sara TengvallAnders Lindén
Mar 21, 2016·Fish & Shellfish Immunology·Chris J Secombes
Mar 24, 2016·Molecular Biology and Evolution·Sudhir KumarKoichiro Tamura
Jun 24, 2016·PLoS Pathogens·Emmanuel Stephen-VictorJagadeesh Bayry
Dec 15, 2016·Fish & Shellfish Immunology·Guoxi LiJianxin Feng